Nucleic Acids Research, 1994, Vol. 22, No. 25 5649-5657
© 1994
Articles |
Repair of a specific double-strand break generated within a mammalian chromosome by yeast endonuclease I-Scel
Department of Biological Sciences, University of South Carolina Columbia, SC 29208, USA
*To whom correspondence should be addressed
Received August 29, 1994. Revised November 12, 1994. Accepted November 4, 1994.
We established a mouse Ltk- cell line that contains within Its genome a herpes simplex virus thymldlne klnase gene (tk) that had been disrupted by the Insertion of the recognition sequence for yeast endonuclease I-Scel. The artificially Introduced 18 bp I-Scel recognition sequence was likely a unique sequence in the genome of the mouse cell line. To assess whether an induced double-strand break (DSB) In the genomlc Mr gene would be repaired preferentially by gene targeting or non-homologous recombination, we electroporated the mouse cell line with endonuclease I-Scel alone, one of two different gene targeting constructs alone, or with I-Scel in conjunction with each of the two targeting constructs. Each targeting construct was, In principle, capable of correcting the defective genomic tk sequence via homologous recombination. tk+ colonies were recovered following electroporatlon of cells with I-Scel In the presence or absence of a targeting construct. Through the detection of small deletions at the I-Scel recognition sequence In the mouse genome, we present evidence that a specific DSB can be Introduced into the genome of a living mammalian cell by yeast endonuclease I-Scel. We further report that a DSB In the genome of a mouse Ltk- cell is repaired preferentially by non-homologous end-joining rather than by targeted homologous recombination with an exogenous donor sequence. The potential utility of this system Is discussed.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Parenteau, M. Durand, S. Veronneau, A.-A. Lacombe, G. Morin, V. Guerin, B. Cecez, J. Gervais-Bird, C.-S. Koh, D. Brunelle, et al. Deletion of Many Yeast Introns Reveals a Minority of Genes that Require Splicing for Function Mol. Biol. Cell, May 1, 2008; 19(5): 1932 - 1941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gottipati, T. N. Cassel, L. Savolainen, and T. Helleday Transcription-Associated Recombination Is Dependent on Replication in Mammalian Cells Mol. Cell. Biol., January 1, 2008; 28(1): 154 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Smith, L. A. Bannister, V. Bhattacharjee, Y. Wang, B. C. Waldman, and A. S. Waldman Accurate Homologous Recombination Is a Prominent Double-Strand Break Repair Pathway in Mammalian Chromosomes and Is Modulated by Mismatch Repair Protein Msh2 Mol. Cell. Biol., November 15, 2007; 27(22): 7816 - 7827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Eklund, U. Y. Ulge, J. Eastberg, and R. J. Monnat Jr Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease Nucleic Acids Res., September 27, 2007; 35(17): 5839 - 5850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Yang, E. B. Goldsmith, Y. Lin, B. C. Waldman, V. Kaza, and A. S. Waldman Genetic Exchange Between Homeologous Sequences in Mammalian Chromosomes Is Averted by Local Homology Requirements for Initiation and Resolution of Recombination Genetics, September 1, 2006; 174(1): 135 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Smith, B. C. Waldman, and A. S. Waldman A Role for DNA Mismatch Repair Protein Msh2 in Error-Prone Double-Strand-Break Repair in Mammalian Chromosomes Genetics, May 1, 2005; 170(1): 355 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Pascucci, M. T. Russo, M. Crescenzi, M. Bignami, and E. Dogliotti The accumulation of MMS-induced single strand breaks in G1 phase is recombinogenic in DNA polymerase {beta} defective mammalian cells Nucleic Acids Res., January 12, 2005; 33(1): 280 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Lee, L. Blanco, T. Zhou, M. Garcia-Diaz, K. Bebenek, T. A. Kunkel, Z. Wang, and L. F. Povirk Implication of DNA Polymerase {lambda} in Alignment-based Gap Filling for Nonhomologous DNA End Joining in Human Nuclear Extracts J. Biol. Chem., January 2, 2004; 279(1): 805 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Manivasakam, J. Aubrecht, S. Sidhom, and R. H. Schiestl Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells Nucleic Acids Res., December 1, 2001; 29(23): 4826 - 4833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lin and A. S. Waldman Promiscuous patching of broken chromosomes in mammalian cells with extrachromosomal DNA Nucleic Acids Res., October 1, 2001; 29(19): 3975 - 3981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lin and A. S. Waldman Capture of DNA Sequences at Double-Strand Breaks in Mammalian Chromosomes Genetics, August 1, 2001; 158(4): 1665 - 1674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Luo, M. A. Macris, A. F. Faruqi, and P. M. Glazer High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells PNAS, July 12, 2000; (2000) 160004997. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Lin, T. Lukacsovich, and A. S. Waldman Multiple Pathways for Repair of DNA Double-Strand Breaks in Mammalian Chromosomes Mol. Cell. Biol., December 1, 1999; 19(12): 8353 - 8360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Waldman, H. Tran, E. C. Goldsmith, and M. A. Resnick Long Inverted Repeats Are an At-Risk Motif for Recombination in Mammalian Cells Genetics, December 1, 1999; 153(4): 1873 - 1883. [Abstract] [Full Text]
|
|
|
|
|
|
T. Lukacsovich and A. S. Waldman Suppression of Intrachromosomal Gene Conversion in Mammalian Cells by Small Degrees of Sequence Divergence Genetics, April 1, 1999; 151(4): 1559 - 1568. [Abstract] [Full Text]
|
|
|
|
|
|
E. Raderschall, E. I. Golub, and T. Haaf Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage PNAS, March 2, 1999; 96(5): 1921 - 1926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Liang, M. Han, P. J. Romanienko, and M. Jasin Homology-directed repair is a major double-strand break repair pathway in mammalian cells PNAS, April 28, 1998; 95(9): 5172 - 5177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cohen-Tannoudji, S. Robine, A. Choulika, D. Pinto, F. El Marjou, C. Babinet, D. Louvard, and F. Jaisser I-SceI-Induced Gene Replacement at a Natural Locus in Embryonic Stem Cells Mol. Cell. Biol., March 1, 1998; 18(3): 1444 - 1448. [Abstract] [Full Text]
|
|
|
|
|
|
B. Elliott, C. Richardson, J. Winderbaum, J. A. Nickoloff, and M. Jasin Gene Conversion Tracts from Double-Strand Break Repair in Mammalian Cells Mol. Cell. Biol., January 1, 1998; 18(1): 93 - 101. [Abstract] [Full Text]
|
|
|
|
|
|
P. Wang, R.-H. Zhou, Y. Zou, C. K. Jackson-Cook, and L. F. Povirk Highly conservative reciprocal translocations formed by apparent joining of exchanged DNA double-strand break ends PNAS, October 28, 1997; 94(22): 12018 - 12023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chu Double Strand Break Repair J. Biol. Chem., September 26, 1997; 272(39): 24097 - 24100. [Full Text] [PDF]
|
|
|
|
|
|
M. E. Moynahan and M. Jasin Loss of heterozygosity induced by a chromosomal double-strand break PNAS, August 19, 1997; 94(17): 8988 - 8993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Liang and M. Jasin Ku80-deficient Cells Exhibit Excess Degradation of Extrachromosomal DNA J. Biol. Chem., June 14, 1996; 271(24): 14405 - 14411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Luo, M. A. Macris, A. F. Faruqi, and P. M. Glazer High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells PNAS, August 1, 2000; 97(16): 9003 - 9008. [Abstract] [Full Text] [PDF]
|
|