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Nucleic Acids Research, 1994, Vol. 22, No. 25 5729-5736
© 1994

Articles

Abundance of nuclear DNA topoisomerase II is correlated with proliferation in Arabidopsis thaliana

Shaoping Xie and Eric Lam1,*

AgBiotech Center, Waksman Institute, Rutgers State University of New Jersey Piscataway, NJ 08854, USA 1Department of Plant Sciences, Waksman Institute, Rutgers State University of New Jersey Piscataway, NJ 08854, USA

*To whom correspondence should be addressed +GenBank L21015; EMBL U12284 and U12285

Received July 19, 1994. Revised November 9, 1994. Accepted November 9, 1994.

Topoisomerase II (TOPII) Is an Important enzyme Involved In DNA replication and chromosome condensation. The level of TOPII expression has been correlated with the prollferatlve state of eukaryotlc cells. Here we report the cloning and characterization of a cDNA clone AtTopll encoding the first reported TOPII from higher plants. AtTopll is 4603 base pairs (bp) in length and encodes an open reading frame of 1473 amlno acid residues. One Interesting feature of AtTopll is the presence of a 110 bp direct repeat In the last one-third of the cDNA. Analysis of the genomic sequence within this region by PCR revealed that this duplication Includes a small Intron of 89 bp. Conservation of sequences within this repeated Intron suggests that this In-frame duplication may be a relatively recent event. The deduced amlno acid sequence of AtTopll shows strong homologles to TOPII sequences reported from other eukaryotes, particularly In the regions that are highly conserved among different species. Southern blot analysis with Arabidopsis DNA indicates that AtTopll Is a single-copy gene while Northern blots detected a 5.0 kb transcript, the level of which is substantially higher in young seedlings than in mature plants. Using a polyclonal antiserum raised against the C-termlnal one-third of AtTOPII, we found that the protein Is localized in the nucleus and its level is correlated with the prollferative state of the particular tissue.


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