Nucleic Acids Research, 1994, Vol. 22, No. 3 434-442
© 1994
MOLECULAR BIOLOGY |
Illegitimate recombination in Xenopus: characterization of end-joined junctions
Department of Biochemistry, University of Utah School of Medicine Salt Lake City, UT 84132, USA
*To whom correspondence should be addressed
Received September 16, 1993. Accepted December 20, 1993.
When linear DNAs are injected into Xenopus laevis eggs, they are converted Into several different kinds of recombination products. Some molecules undergo homologous recombination by a resection-annealing mechanism; some ends are precisely llgated; and some ends are Joined by illegitimate means. The homologous and illegitimate products are also generated in nuclear extracts from stage VI Xenopus oocytes. In order to gain insight Into the mechanism(s) of illegitimate end joining, we amplified, cloned and sequenced a number of junctions from eggs and from oocyte extracts. The egg junctions fell into three categories: some with no homology at the join point that may have been produced by blunt-end ligation; some based on small, but significant homologies (5–10 bp); and some with matches of only 1 or 2 nucleotides at the joint. Junctions made in oocyte extracts were largely of the latter type. In the extracts, formation of illegitimate joints required the addition of all four deoxyribo-nucleoside triphosphates and was inhibited by aphidl-colin. This indicates that this process involves DNA synthesis, and mechanisms incorporating this feature are considered. The spectrum of recombination products formed in Xenopus eggs is very reminiscent of those produced from DNA introduced into mammalian cells.
+Present address: Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720, USA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Pacher, W. Schmidt-Puchta, and H. Puchta Two Unlinked Double-Strand Breaks Can Induce Reciprocal Exchanges in Plant Genomes via Homologous Recombination and Nonhomologous End Joining Genetics, January 1, 2007; 175(1): 21 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Morton, M. W. Davis, E. M. Jorgensen, and D. Carroll Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells PNAS, October 31, 2006; 103(44): 16370 - 16375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lloyd, C. L. Plaisier, D. Carroll, and G. N. Drews Targeted mutagenesis using zinc-finger nucleases in Arabidopsis PNAS, February 8, 2005; 102(6): 2232 - 2237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Puchta The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution J. Exp. Bot., January 1, 2005; 56(409): 1 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Siebert and H. Puchta Efficient Repair of Genomic Double-Strand Breaks by Homologous Recombination between Directly Repeated Sequences in the Plant Genome PLANT CELL, May 1, 2002; 14(5): 1121 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Pospiech, A. K. Rytkonen, and J. E. Syvaoja The role of DNA polymerase activity in human non-homologous end joining Nucleic Acids Res., August 1, 2001; 29(15): 3277 - 3288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Onda, J. Yamaguchi, K. Hanada, Y. Asami, and H. Ikeda Role of DNA Ligase in the Illegitimate Recombination That Generates {{lambda}}bio-Transducing Phages in Escherichia coli Genetics, May 1, 2001; 158(1): 29 - 39. [Abstract] [Full Text]
|
|
|
|
|
|
E. Feldmann, V. Schmiemann, W. Goedecke, S. Reichenberger, and P. Pfeiffer DNA double-strand break repair in cell-free extracts from Ku80-deficient cells: implications for Ku serving as an alignment factor in non-homologous DNA end joining Nucleic Acids Res., July 1, 2000; 28(13): 2585 - 2596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pfeiffer, W. Goedecke, and G. Obe Mechanisms of DNA double-strand break repair and their potential to induce chromosomal aberrations Mutagenesis, July 1, 2000; 15(4): 289 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Schrader, W. Edelmann, R. Kucherlapati, and J. Stavnezer Reduced Isotype Switching in Splenic B Cells from Mice Deficient in Mismatch Repair Enzymes J. Exp. Med., August 2, 1999; 190(3): 323 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. King, C. F. Fairley, and W. F. Morgan DNA End Joining by the Klenow Fragment of DNA Polymerase I J. Biol. Chem., August 23, 1996; 271(34): 20450 - 20457. [Abstract] [Full Text] [PDF]
|
|