Skip Navigation

This Article
Right arrow Print PDF (7537K)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Itoh-Lindstrom, Y.
Right arrow Articles by Leffak, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Itoh-Lindstrom, Y.
Right arrow Articles by Leffak, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 3 498-505
© 1994

MOLECULAR BIOLOGY

Alteration of in vivo DNA synthesis in the alpha globin locus of chick embryo fibroblasts due to in vivo activity of Rous sarcoma virus pp60src

Yoshie Itoh-Lindstrom1,+ and Michael Leffak1,2,*

1Department of Biochemistry and Molecular Biology, Wright State University Dayton OH 45435 2Kettering Medical Center - Wright State University Clinical Molecular Genetics Laboratory Dayton OH 45435, USA

*To whom correspondence should be addressed

Received August 18, 1993. Accepted January 4, 1994.

Globin gene transcription is activated in chick embryo fibroblasts (CEF) transformed by Rous sarcoma virus (RSV). To determine whether this activation is correlated with a change in the replication of the alphaD giobin locus we used a nuclear runoff replication assay. Density iabeiing of nuclei showed that repilcatlon of the aiphaD giobin gene in normai and RSV-transformed CEF (RSV-CEF) is in the transcrlptionai direction. However, in RSV-CEF the signai from a specific restriction fragment between the aiphaD and alphaA giobin genes was lower in the replicated DNA. This decrease was selective in that the signal of this restriction fragment was not diminished in the unrepiicated DNA In the same nuclei. When CEF transformed by temperature sensitive RSV were grown at the restrictive temperature for pp60arc activity the loss of transformed cell morphology correlated with the reappear ance of the replicated alpha giobin DNA fragments in their normal stoichiometry. By comparison, histone H5 gene expression was not seen in CEF or in RSV-CEF, and H5 gene replication was not perturbed by viral transformation. These results suggest that the viral pp60arc protein can activate transcription of the aiphaD giobin gene and change the replication of alpha giobln chromatin.

+Present address: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
D. Di Paola, G. B. Price, and M. Zannis-Hadjopoulos
Differentially Active Origins of DNA Replication in Tumor versus Normal Cells.
Cancer Res., May 15, 2006; 66(10): 5094 - 5103.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.