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Nucleic Acids Research, 1994, Vol. 22, No. 5 703-710
© 1994

CHEMISTRY

Incorporation of (R)- and (S)-3',4'-seco-thymidine into oligodeoxynucleotides: hybridization properties and enzymatic stability

Poul Nielsen, Finn Kirpekar1 and Jesper Wengel*

Department of Chemistry, Odense University DK-5230 Odense M, Denmark 1Department of Molecular Biology, Odense University DK-5230 Odense M, Denmark

*To whom correspondence should be addressed

Received January 4, 1994. Accepted February 7, 1994.

Novel flexible ollgodeoxynucleotide analogues containing (R)- and (S)-3',4'-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidlte approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications In the middle or one or two modifications In the ends were evaluated with respect to hybridization properties and enzymatic stability. 3'-End-modlfled oligomers were stable towards 3'-exonuclease degradation and displayed acceptable hybridization properties.


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