Nucleic Acids Research, 1994, Vol. 22, No. 6 965-971
© 1994
MOLECULAR BIOLOGY |
Recognition sequence of a highly conserved DNA binding protein RBP-Jx
Department of Medical Chemistry, Kyoto University Faculty of Medicine Yoshida, Sakyo-ku, Kyoto 606, Japan
*To whom correspondence should be addressed at: Nara Institute of Science and Technology 8916-5. Takayama-cho. Ikoma 630-01. Japan
Received January 4, 1994. Revised January 18, 1994. Accepted January 18, 1994.
DNA binding specificity of the RBP-Jx protein was extensively examined. The mouse RBP-Jx protein was originally isolated as a nuclear protein binding to the Jx type V(D)J recombination signal sequence which consisted of the conserved heptamer (CACTGTG) and nonamer (GGTTTTTGT) sequences separated by a 23-base pair spacer. Electrophoretic mobility shift assay using DNA probes with mutations In various parts of the Jx recombination signal sequence showed that the RBP-Jx protein recognized the sequence outside the recombination signal in addition to the heptamer but did not recognize the nonamer sequence and the spacer length at all. Database search identified the best naturally occurring binding motif (CACTGTGGGAA-CGG) for the RBP-Jx protein in the promoter region of the m8 gene in the Enhancer of split gene cluster of Drosophila. The binding assay with a series of m8 motif mutants indicated that the protein recognized mostly the GTGGGAA sequence and also interacted weakly with ACT and CG sequences flanking this hepta-nucleotide. Oligonucleotides binding to the RBP-Jx protein were enriched from a pool of synthetic oligonucleotides containing 20-base random sequences by the repeated electrophoretic mobility shift assay. The enriched ollgomer shared a common sequence of CGTGGGAA. All these data indicate that the RBP-Jx protein recognizes a unique core sequence of CGTGGGAA and does not bind to the V(D)J recombination signal without the flanking sequence.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. C. Pinnix, J. T. Lee, Z.-J. Liu, R. McDaid, K. Balint, L. J. Beverly, P. A. Brafford, M. Xiao, B. Himes, S. E. Zabierowski, et al. Active Notch1 Confers a Transformed Phenotype to Primary Human Melanocytes Cancer Res., July 1, 2009; 69(13): 5312 - 5320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Scheinman and O. Avni Transcriptional Regulation of Gata3 in T Helper Cells by the Integrated Activities of Transcription Factors Downstream of the Interleukin-4 Receptor and T Cell Receptor J. Biol. Chem., January 30, 2009; 284(5): 3037 - 3048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Sivasankaran, M. Degen, A. Ghaffari, M. E. Hegi, M.-F. Hamou, M.-C. S. Ionescu, C. Zweifel, M. Tolnay, M. Wasner, S. Mergenthaler, et al. Tenascin-C Is a Novel RBPJ{kappa}-Induced Target Gene for Notch Signaling in Gliomas Cancer Res., January 15, 2009; 69(2): 458 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ogino, M. Fisher, and R. M. Grainger Convergence of a head-field selector Otx2 and Notch signaling: a mechanism for lens specification Development, January 15, 2008; 135(2): 249 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Singh, B. Voleti, and A. Agrawal A Novel RBP-J{kappa}-Dependent Switch from C/EBPbeta to C/EBP{zeta} at the C/EBP Binding Site on the C-Reactive Protein Promoter J. Immunol., June 1, 2007; 178(11): 7302 - 7309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nam, P. Sliz, W. S. Pear, J. C. Aster, and S. C. Blacklow Cooperative assembly of higher-order Notch complexes functions as a switch to induce transcription PNAS, February 13, 2007; 104(7): 2103 - 2108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Pegman, S. M. Smith, B. N. D'Souza, S. T. Loughran, S. Maier, B. Kempkes, P. A. Cahill, M. J. Simmons, C. Gelinas, and D. Walls Epstein-Barr Virus Nuclear Antigen 2 trans-Activates the Cellular Antiapoptotic bfl-1 Gene by a CBF1/RBPJ{kappa}-Dependent Pathway. J. Virol., August 1, 2006; 80(16): 8133 - 8144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Weng, J. M. Millholland, Y. Yashiro-Ohtani, M. L. Arcangeli, A. Lau, C. Wai, C. del Bianco, C. G. Rodriguez, H. Sai, J. Tobias, et al. c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphoma Genes & Dev., August 1, 2006; 20(15): 2096 - 2109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Beres, T. Masui, G. H. Swift, L. Shi, R. M. Henke, and R. J. MacDonald PTF1 Is an Organ-Specific and Notch-Independent Basic Helix-Loop-Helix Complex Containing the Mammalian Suppressor of Hairless (RBP-J) or Its Paralogue, RBP-L Mol. Cell. Biol., January 1, 2006; 26(1): 117 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lamar and C. Kintner The Notch targets Esr1 and Esr10 are differentially regulated in Xenopus neural precursors Development, August 15, 2005; 132(16): 3619 - 3630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Sarmento, H. Huang, A. Limon, W. Gordon, J. Fernandes, M. J. Tavares, L. Miele, A. A. Cardoso, M. Classon, and N. Carlesso Notch1 modulates timing of G1-S progression by inducing SKP2 transcription and p27Kip1 degradation J. Exp. Med., July 5, 2005; 202(1): 157 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Anthony, H. A. Mason, T. Gridley, G. Fishell, and N. Heintz Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells Genes & Dev., May 1, 2005; 19(9): 1028 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lan, D. A. Kuppers, and E. S. Robertson Kaposi's Sarcoma-Associated Herpesvirus Reactivation Is Regulated by Interaction of Latency-Associated Nuclear Antigen with Recombination Signal Sequence-Binding Protein J{kappa}, the Major Downstream Effector of the Notch Signaling Pathway J. Virol., March 15, 2005; 79(6): 3468 - 3478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, J. Huang, F. Y. Wu, G. Liao, L. Hutt-Fletcher, and S. D. Hayward Regulation of Expression of the Epstein-Barr Virus BamHI-A Rightward Transcripts J. Virol., February 1, 2005; 79(3): 1724 - 1733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liang and D. Ganem RBP-J (CSL) Is Essential for Activation of the K14/vGPCR Promoter of Kaposi's Sarcoma-Associated Herpesvirus by the Lytic Switch Protein RTA J. Virol., July 1, 2004; 78(13): 6818 - 6826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lim, H. Kim, and K. H. Kim The Ku Antigen-Recombination Signal-binding Protein J{kappa} Complex Binds to the Nuclear Factor-{kappa}B p50 Promoter and Acts as a Positive Regulator of p50 Expression in Human Gastric Cancer Cells J. Biol. Chem., January 2, 2004; 279(1): 231 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nam, A. P. Weng, J. C. Aster, and S. C. Blacklow Structural Requirements for Assembly of the CSL{middle dot}Intracellular Notch1{middle dot}Mastermind-like 1 Transcriptional Activation Complex J. Biol. Chem., May 30, 2003; 278(23): 21232 - 21239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. T. Krebs, N. Iwai, S. Nonaka, I. C. Welsh, Y. Lan, R. Jiang, Y. Saijoh, T. P. O'Brien, H. Hamada, and T. Gridley Notch signaling regulates left-right asymmetry determination by inducing Nodal expression Genes & Dev., May 15, 2003; 17(10): 1207 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Vales and E. M. Friedl Binding of C/EBP and RBP (CBF1) to Overlapping Sites Regulates Interleukin-6 Gene Expression J. Biol. Chem., November 1, 2002; 277(45): 42438 - 42446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Lee, K.-H. Lee, M. Weidner, B. A. Osborne, and S. D. Hayward Epstein-Barr virus EBNA2 blocks Nur77- mediated apoptosis PNAS, September 3, 2002; 99(18): 11878 - 11883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liang, J. Chang, S. J. Lynch, D. M. Lukac, and D. Ganem The lytic switch protein of KSHV activates gene expression via functional interaction with RBP-Jkappa (CSL), the target of the Notch signaling pathway Genes & Dev., August 1, 2002; 16(15): 1977 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rebeiz, N. L. Reeves, and J. W. Posakony SCORE: A computational approach to the identification of cis-regulatory modules and target genes in whole-genome sequence data PNAS, July 23, 2002; 99(15): 9888 - 9893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Fennewald, J. F. Aronson, L. Zhang, and N. K. Herzog Alterations in NF-{kappa}B and RBP-J{kappa} by Arenavirus Infection of Macrophages In Vitro and In Vivo J. Virol., February 1, 2002; 76(3): 1154 - 1162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Reizis and P. Leder Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway Genes & Dev., February 1, 2002; 16(3): 295 - 300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-D. Wang, G.-D. Chang, Y.-K. Lee, and H. Chen A Functional Composite Cis-Element for NF{kappa}B and RBPJ{kappa} in the Rat Pregnancy-Specific Glycoprotein Gene Biol Reprod, November 1, 2001; 65(5): 1437 - 1443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhou and S. D. Hayward Nuclear Localization of CBF1 Is Regulated by Interactions with the SMRT Corepressor Complex Mol. Cell. Biol., September 15, 2001; 21(18): 6222 - 6232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Gordadze, R. Peng, J. Tan, G. Liu, R. Sutton, B. Kempkes, G. W. Bornkamm, and P. D. Ling Notch1IC Partially Replaces EBNA2 Function in B Cells Immortalized by Epstein-Barr Virus J. Virol., July 1, 2001; 75(13): 5899 - 5912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Tang and T. Kadesch Identification of a novel activation domain in the Notch-responsive transcription factor CSL Nucleic Acids Res., June 1, 2001; 29(11): 2284 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Doyle, C. Wen, and I. Greenwald SEL-8, a nuclear protein required for LIN-12 and GLP-1 signaling in Caenorhabditis elegans PNAS, July 5, 2000; 97(14): 7877 - 7881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jeffries and A. J. Capobianco Neoplastic Transformation by Notch Requires Nuclear Localization Mol. Cell. Biol., June 1, 2000; 20(11): 3928 - 3941. [Abstract] [Full Text]
|
|
|
|
|
|
S. H. Lee, X.-l. Wang, and J. DeJong Functional interactions between an atypical NF-{kappa}B site from the rat CYP2B1 promoter and the transcriptional repressor RBP-J{kappa}/CBF1 Nucleic Acids Res., May 15, 2000; 28(10): 2091 - 2098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhou, M. Fujimuro, J. J.-D. Hsieh, L. Chen, A. Miyamoto, G. Weinmaster, and S. D. Hayward SKIP, a CBF1-Associated Protein, Interacts with the Ankyrin Repeat Domain of NotchIC To Facilitate NotchIC Function Mol. Cell. Biol., April 1, 2000; 20(7): 2400 - 2410. [Abstract] [Full Text]
|
|
|
|
|
|
S. Zhou, M. Fujimuro, J. J.-D. Hsieh, L. Chen, and S. D. Hayward A Role for SKIP in EBNA2 Activation of CBF1-Repressed Promoters J. Virol., February 15, 2000; 74(4): 1939 - 1947. [Abstract] [Full Text]
|
|
|
|
|
|
V. Morel and F. Schweisguth Repression by Suppressor of Hairless and activation by Notch are required to define a single row of single-minded expressing cells in the Drosophila embryo Genes & Dev., February 1, 2000; 14(3): 377 - 388. [Abstract] [Full Text]
|
|
|
|
|
|
E. Lai, R Bodner, and J. Posakony The enhancer of split complex of Drosophila includes four Notch-regulated members of the bearded gene family Development, January 8, 2000; 127(16): 3441 - 3455. [Abstract] [PDF]
|
|
|
|
|
|
E. Lai, R Bodner, J Kavaler, G Freschi, and J. Posakony Antagonism of notch signaling activity by members of a novel protein family encoded by the bearded and enhancer of split gene complexes Development, January 1, 2000; 127(2): 291 - 306. [Abstract] [PDF]
|
|
|
|
 |
|
 K. G. Krauer, M. Buck, and T. Sculley Characterization of the transcriptional repressor RBP in Epstein-Barr virus-transformed B cells J. Gen. Virol., December 1, 1999; 80(12): 3217 - 3226. [Abstract] [Full Text]
|
|
|
|
|
|
H Takahashi, Y Mitani, G Satoh, and N Satoh Evolutionary alterations of the minimal promoter for notochord-specific Brachyury expression in ascidian embryos Development, January 9, 1999; 126(17): 3725 - 3734. [Abstract] [PDF]
|
|
|
|
|
|
J. J.-D. Hsieh, S. Zhou, L. Chen, D. B. Young, and S. D. Hayward CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex PNAS, January 5, 1999; 96(1): 23 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jarriault, O. Le Bail, E. Hirsinger, O. Pourquié, F. Logeat, C. F. Strong, C. Brou, N. G. Seidah, and A. Israël Delta-1 Activation of Notch-1 Signaling Results in HES-1 Transactivation Mol. Cell. Biol., December 1, 1998; 18(12): 7423 - 7431. [Abstract] [Full Text]
|
|
|
|
|
|
L. T. Lam and E. H. Bresnick Identity of the beta -Globin Locus Control Region Binding Protein HS2NF5 as the Mammalian Homolog of the Notch-regulated Transcription Factor Suppressor of Hairless J. Biol. Chem., September 11, 1998; 273(37): 24223 - 24231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sakai, Y. Taniguchi, K. Tamura, S. Minoguchi, T. Fukuhara, L. J. Strobl, U. Zimber-Strobl, G. W. Bornkamm, and T. Honjo Functional Replacement of the Intracellular Region of the Notch1 Receptor by Epstein-Barr Virus Nuclear Antigen 2 J. Virol., July 1, 1998; 72(7): 6034 - 6039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Olave, D. Reinberg, and L. D. Vales The mammalian transcriptional repressor RBP (CBF1) targets TFIID and TFIIA to prevent activated transcription Genes & Dev., June 1, 1998; 12(11): 1621 - 1637. [Abstract] [Full Text]
|
|
|
|
|
|
F. Oswald, S. Liptay, G. Adler, and R. M. Schmid NF-kappa B2 Is a Putative Target Gene of Activated Notch-1 via RBP-Jkappa Mol. Cell. Biol., April 1, 1998; 18(4): 2077 - 2088. [Abstract] [Full Text]
|
|
|
|
|
|
K. Miyazawa, A. Mori, K. Yamamoto, and H. Okudaira Transcriptional Roles of CCAAT/Enhancer Binding Protein-beta , Nuclear Factor-kappa B, and C-promoter Binding Factor 1 in Interleukin (IL)-1beta -induced IL-6 Synthesis by Human Rheumatoid Fibroblast-like Synoviocytes J. Biol. Chem., March 27, 1998; 273(13): 7620 - 7627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Taniguchi, T. Furukawa, T. Tun, H. Han, and T. Honjo LIM Protein KyoT2 Negatively Regulates Transcription by Association with the RBP-J DNA-Binding Protein Mol. Cell. Biol., January 1, 1998; 18(1): 644 - 654. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Chen, W. H. Fischer, and G. N. Gill Regulation of the ERBB-2 Promoter by RBPJkappa and NOTCH J. Biol. Chem., May 30, 1997; 272(22): 14110 - 14114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Kato, Y Taniguchi, H Kurooka, S Minoguchi, T Sakai, S Nomura-Okazaki, K Tamura, and T Honjo Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives Development, January 10, 1997; 124(20): 4133 - 4141. [Abstract] [PDF]
|
|
|
|
|
|
J. Corbo, M Levine, and R. Zeller Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis Development, January 2, 1997; 124(3): 589 - 602. [Abstract] [PDF]
|
|
|
|
|
|
D. Wettstein, D. Turner, and C Kintner The Xenopus homolog of Drosophila Suppressor of Hairless mediates Notch signaling during primary neurogenesis Development, January 2, 1997; 124(3): 693 - 702. [Abstract] [PDF]
|
|
|
|
|
|
S Christensen, V Kodoyianni, M Bosenberg, L Friedman, and J Kimble lag-1, a gene required for lin-12 and glp-1 signaling in Caenorhabditis elegans, is homologous to human CBF1 and Drosophila Su(H) Development, January 5, 1996; 122(5): 1373 - 1383. [Abstract] [PDF]
|
|
|
|
|
|
P Heitzler, M Bourouis, L Ruel, C Carteret, and P Simpson Genes of the Enhancer of split and achaete-scute complexes are required for a regulatory loop between Notch and Delta during lateral signalling in Drosophila Development, January 1, 1996; 122(1): 161 - 171. [Abstract] [PDF]
|
|
|
|
|
|
M Lecourtois and F Schweisguth The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling. Genes & Dev., November 1, 1995; 9(21): 2598 - 2608. [Abstract] [PDF]
|
|
|
|
|
|
A M Bailey and J W Posakony Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity. Genes & Dev., November 1, 1995; 9(21): 2609 - 2622. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Hsieh and S. Hayward Masking of the CBF1/RBPJ kappa transcriptional repression domain by Epstein-Barr virus EBNA2 Science, April 28, 1995; 268(5210): 560 - 563. [Abstract] [PDF]
|
|
|
|
|
|
S Artavanis-Tsakonas, K Matsuno, and M. Fortini Notch signaling Science, April 14, 1995; 268(5208): 225 - 232. [Abstract] [PDF]
|
|
|
|
|
|
C Oka, T Nakano, A Wakeham, J. de la Pompa, C Mori, T Sakai, S Okazaki, M Kawaichi, K Shiota, T. Mak, et al. Disruption of the mouse RBP-J kappa gene results in early embryonic death Development, January 10, 1995; 121(10): 3291 - 3301. [Abstract] [PDF]
|
|
|
|
|
|
F Schweisguth Suppressor of Hairless is required for signal reception during lateral inhibition in the Drosophila pupal notum Development, January 6, 1995; 121(6): 1875 - 1884. [Abstract] [PDF]
|
|
|
|
|
|
C Brou, F Logeat, M Lecourtois, J Vandekerckhove, P Kourilsky, F Schweisguth, and A Israel Inhibition of the DNA-binding activity of Drosophila suppressor of hairless and of its human homolog, KBF2/RBP-J kappa, by direct protein-protein interaction with Drosophila hairless. Genes & Dev., October 15, 1994; 8(20): 2491 - 2503. [Abstract] [PDF]
|
|
|
|
|
|
A Singson, M W Leviten, A G Bang, X H Hua, and J W Posakony Direct downstream targets of proneural activators in the imaginal disc include genes involved in lateral inhibitory signaling. Genes & Dev., September 1, 1994; 8(17): 2058 - 2071. [Abstract] [PDF]
|
|
|
|
|
|
T Henkel, P. Ling, S. Hayward, and M. Peterson Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein J kappa Science, July 1, 1994; 265(5168): 92 - 95. [Abstract] [PDF]
|
|