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Nucleic Acids Research, 1994, Vol. 22, No. 7 1138-1146
© 1994

MOLECULAR BIOLOGY

A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene

Michael A. Hollingsworth*, Christina Closken, Ann Harris1, Claudia D. McDonald, Gurcharan S. Pahwa and L.James Maher, III

Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center Omaha, NE 68198-6805, USA 1Paediatric Molecular Genetics, Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital Oxford 0X3 9DU, UK

*To whom correspondence should be addressed

Received January 27, 1994. Accepted February 28, 1994.

We have identified two regions of non-random purine/pyrimldine strand asymmetry that were nearly identical in sequence in the 5' flanking (promoter) regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene and the human MUC1 gene. These regions contain perfect mirror repeat elements, a sequence motif previously found to be associated with the formation of H-DNA conformations. In this report we demonstrate that a single-stranded non-B DNA conformation exists at low pH in supercoiled plasmids containing the similar mirror repeat elements, and that S1 nuclease digestion maps the single-stranded region to the position of the mirror repeats. In addition, we identify a nuclear protein of approximately 27 kD that binds to single-stranded oligonucleotides corresponding to the purtne-rlch strand of this region, but not to the pyrlmldlne-rlch strands or to double-stranded oligonucleotides with corresponding purine/pyrimidlne strand asymmetry.


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