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Nucleic Acids Research, 1994, Vol. 22, No. 7 1161-1166
© 1994

MOLECULAR BIOLOGY

Human androgen receptor expressed in HeLa cells activates transcription in vitro

Piet De Vos, Jacky Schmitt1,+, Guido Verhoeven* and Hendrik G. Stunnenberg1

Legendo, University of Leuven, Faculty of Medicine Gasthuisberg, B-3000 Leuven, Belgium 1European Molecular Biology Laboratory, Gene Expression Programme Meyerhofstrasse 1, 69118 Heidelberg, Germany

*To whom correspondence should be addressed

Received January 7, 1994. Revised February 23, 1994. Accepted February 23, 1994.

The androgen receptor (AR) is a ligand-responsive transcription factor, belonging to the class of steroid receptors. AR mutations have been associated with various X-llnked diseases, characterized by complete or partial resistance to androgens. To further analyse the molecular mechanism of action of the AR, we have produced the human AR in HeLa cells with a Vaccinia virus expression system. Binding studies on infected HeLa cells demonstrate that the recombinant AR interacts specifically and with high affinity with natural and synthetic androgens. In a gel retardation assay the partially purified AR specifically recognizes an androgen response element of the rat prostatic binding protein gene. Moreover, the recombinant AR activates transcription in vitro from a synthetic promoter construct containing glucocortlcold response elements (GRE).

+ Present address: Max-Planck Institut fur Immunbiologie, Stübeweg 51. 79108 Freiburg, Germany


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