Nucleic Acids Research, 1994, Vol. 22, No. 7 1202-1207
© 1994
MOLECULAR BIOLOGY |
A distinct class of homeodomain proteins is encoded by two sequentially expressed Drosophila genes from the 93D/E cluster
Laboratoire de Génétique Moléculaire des Eukaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de I'INSERM, Institut de Chimie Biologique, Faculté de Médecine 11 rue Humann, 67085 Strasbourg Cedex, France
*To whom correspondence should be addressed
Received December 22, 1993. Revised February 23, 1994. Accepted February 23, 1994.
Homeodomains appear to be one of the most frequently employed DNA-blndlng domains in a superfamily of transacting factors. It is likely that during evolution several sub-types of homeodomain have evolved from a common ancestral domain, resulting in distinct but closely related DNA-blnding preferences. Here we describe the conservation of a distinct type of homeodomain encoded by the Drosophila lady-bird-late (Ibl) gene, previously named nkch4 (1). Using degenerate PCR primers corresponding to the most divergent regions of the first and third helix of the Lbl homeodomain we have amplified, from genomic DNA of the fly, a lady-blrd-Uke homeobox fragment. The Drosophila PCR products contained both the lbl (1) and a highly related homeobox sequence, which we named ladybird-early (Ibe). This new Drosophila gene resides directly upstream to Ibl and together with tinman/NK4 (2, 3, 4, 5), bagplpe/NK3 (2, 4) S59/NK1 (4, 6) and 93Bal (7) compose the 93D/E homeobox gene cluster. Ibe and Ibl are transcribed from the same strand and In a temporal order corresponding to their 5' – 3' chromosomal location. Transcripts of both genes are found in the eplderm of Drosophila embryos, in cells known to express a segment polarity gene wingless (8), and their spatial and temporal collnearity of expression strongly suggests that they cooperate during segmentation. The amlno-acid composition of both Lady-bird homeo-domains differ from that of Antp-type at several positions involved in DNA recognition. These substitutions appear to modify DNA-binding preferences since Lbl homeodomain is unable to recognize the most common homeodomain binding TAAT motif in gel retardation experiments.
+Present address: Institut fur Zellbiologie, ETH-Honggerberg. CH-8093 Zurich, Switzerland
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Rogulja-Ortmann, K. Luer, J. Seibert, C. Rickert, and G. M. Technau Programmed cell death in the embryonic central nervous system of Drosophila melanogaster Development, January 1, 2007; 134(1): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sato, K. Kitahara, T. Susa, T. Kato, and Y. Kato Pituitary transcription factor Prop-1 stimulates porcine pituitary glycoprotein hormone {alpha} subunit gene expression. J. Mol. Endocrinol., October 1, 2006; 37(2): 341 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Urbach and G. M. Technau Molecular markers for identified neuroblasts in the developing brain of Drosophila Development, August 15, 2003; 130(16): 3621 - 3637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Brohmann, K Jagla, and C Birchmeier The role of Lbx1 in migration of muscle precursor cells Development, January 1, 2000; 127(2): 437 - 445. [Abstract] [PDF]
|
|
|
|
|
|
T Jagla, F Bellard, Y Lutz, G Dretzen, M Bellard, and K Jagla ladybird determines cell fate decisions during diversification of Drosophila somatic muscles Development, January 9, 1998; 125(18): 3699 - 3708. [Abstract] [PDF]
|
|
|
|
|
|
S Dietrich, F. Schubert, C Healy, P. Sharpe, and A Lumsden Specification of the hypaxial musculature Development, January 6, 1998; 125(12): 2235 - 2249. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. M. Lee, T. Park, R. A. Schulz, and Y. Kim Twist-mediated Activation of the NK-4 Homeobox Gene in the Visceral Mesoderm of Drosophila Requires Two Distinct Clusters of E-box Regulatory Elements J. Biol. Chem., July 11, 1997; 272(28): 17531 - 17541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Jagla, M Frasch, T Jagla, G Dretzen, F Bellard, and M Bellard ladybird, a new component of the cardiogenic pathway in Drosophila required for diversification of heart precursors Development, January 9, 1997; 124(18): 3471 - 3479. [Abstract] [PDF]
|
|
|
|
|
|
K Jagla, T Jagla, P Heitzler, G Dretzen, F Bellard, and M Bellard ladybird, a tandem of homeobox genes that maintain late wingless expression in terminal and dorsal epidermis of the Drosophila embryo Development, January 1, 1997; 124(1): 91 - 100. [Abstract] [PDF]
|
|
|
|
|
|
D Onichtchouk, V Gawantka, R Dosch, H Delius, K Hirschfeld, C Blumenstock, and C Niehrs The Xvent-2 homeobox gene is part of the BMP-4 signalling pathway controlling [correction of controling] dorsoventral patterning of Xenopus mesoderm Development, January 10, 1996; 122(10): 3045 - 3053. [Abstract] [PDF]
|
|