Skip Navigation

This Article
Right arrow Print PDF (5962K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cheng, S.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, S.-C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 9 1548-1554
© 1994

RNA

Formation of the yeast splicing complex A1 and association of the splicing factor PRP19 with the pre-mRNA are independent of the 3' region of the intron

Soo-Chen Cheng

Institute of Molecular Biology, Academia Sinica Nankang, Taiwan, ROC

Received February 24, 1994. Revised April 8, 1994. Accepted April 8, 1994.

Assembly of the spliceosome is a step-wise process and involves squential binding of snRNAs to the pre-mRNA to form pre-splicing complex A2-1. Subsequent dissociation of U4 from the spliceosome is accompanied by formation of complex A1 (Genes Dev. 1, 1014–1027, 1987). We show that the 3' region of the intron sequence is not required for efficient assembly of the yeast spliceosome. Truncated precursor mRNA retaining only four or five nucleotides 3' to the TACTAAC box formed pre-splicing complex A1, kinetically the last pre-mRNA containing splicing complex identified. The subsequent cleavage–ligation reaction requires at least 23 nucleotides on the 3' side of the TACTAAC box in a sequence-independent manner. Immunoprecipitation with anti-PRP19 antibody showed that association of PRP19 with the spliceosome was also independent of the 3' region of the intron.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. C. McGrail, A. Krause, and R. T. O'Keefe
The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing
Nucleic Acids Res., July 1, 2009; 37(13): 4205 - 4217.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R.-T. Tsai, R.-H. Fu, F.-L. Yeh, C.-K. Tseng, Y.-C. Lin, Y.-h. Huang, and S.-C. Cheng
Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2
Genes & Dev., December 15, 2005; 19(24): 2991 - 3003.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-P. Chan and S.-C. Cheng
The Prp19-associated Complex Is Required for Specifying Interactions of U5 and U6 with Pre-mRNA during Spliceosome Activation
J. Biol. Chem., September 2, 2005; 280(35): 31190 - 31199.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S.-P. Chan, D.-I Kao, W.-Y. Tsai, and S.-C. Cheng
The Prp19p-Associated Complex in Spliceosome Activation
Science, October 10, 2003; 302(5643): 279 - 282.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. H. McDonald, R. Ohi, N. Smelkova, D. Frendewey, and K. L. Gould
Myb-Related Fission Yeast cdc5p Is a Component of a 40S snRNP-Containing Complex and Is Essential for Pre-mRNA Splicing
Mol. Cell. Biol., August 1, 1999; 19(8): 5352 - 5362.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.