Skip Navigation

This Article
Right arrow Print PDF (7521K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Schweizer-Groyer, G.
Right arrow Articles by Pictet, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schweizer-Groyer, G.
Right arrow Articles by Pictet, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 9 1583-1592
© 1994

MOLECULAR BIOLOGY

Expression from the tyrosine aminotransferase promoter (nt –350 to +1) is liver-specific and dependent on the binding of both liver-enriched and ubiquitous trans-acting factors

Ghislaine Schweizer-Groyer1,*, André Groyer+,, Françoise Cadepond1, Thierry Grange, Etienne-Emile Baulieu1 and Raymond Pictet

CNRS Institut Jacques Monod and INSERM U 257, Couloir 42–43, 2 Place Jussieu, 75251 Paris 1INSERM U 33, 80 rue du Général Leclerc, 94276 Le Kremlin-Bicêtre Cedex, France

*To whom correspondence should be addressed

Received February 10, 1994. Revised March 24, 1994. Accepted March 24, 1994.

The rat tyrosine aminotransferase (TAT) gene promoter (nucleotides – 350 to +1; TAT0.35) was able to sustain liver-specific expression both ex vivo in transient transfection (TAT-expressing H4IIEC3 hepatoma cells vs. TAT non-expressing CCL1.2 fibroblasts) and in in vitro transcription (rat liver vs. spleen crude nuclear extracts). In either case, the index of tissue specificity (6.2 and 6.7 in ex vivo and in vitro experiments, respectively) was close to that obtained with 10 Kb of TAT gene 5'-flanking sequences in transient transfection. Using computer-assisted search of homologies, DNase I footprinting, gel retardation and methylation interference assays, we showed that TAT0.35 sequences spanning nt –156 to –175 and nt –268 to –281 interacted with the liver enriched NF-1Liver (a member of the NF1 gene family) and HNF1 respectively, whereas those encompassing nt –57 to –85 and nt –283 to –288 interacted with the ubiquitous NF-Y and with ubiquitous ‘CCAAT’-box binding factor(s), respectively. Competition studies in in vitro transcription carried out with wild type and mutated oligonucleotides, demonstrated that NF-Y cis-elements were crucial for basal TAT promoter activity, both in liver and spleen whereas NF1Liver and HNF1 were only efficient in the liver (supported ~60% and 30% of basal TAT0.35 activity respectively). Altogether, these results support the conclusion that TAT0.35 was able to sustain at least part of the liver specificity of TAT gene expression.

+Present address: INSERM U142, Hôpital Saint Antoine, 75571 Paris Cedex 12, France


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y. Sato, K. Miyake, H. Kaneoka, and S. Iijima
Sumoylation of CCAAT/Enhancer-binding Protein {alpha} and Its Functional Roles in Hepatocyte Differentiation
J. Biol. Chem., August 4, 2006; 281(31): 21629 - 21639.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
S. R. Leib, T. C. McGuire, and D. J. Prieur
Comparison of the Tyrosine Aminotransferase cDNA and Genomic DNA Sequences of Normal Mink and Mink Affected with Tyrosinemia Type II
J. Hered., July 1, 2005; 96(4): 302 - 309.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Liberati, A. Ronchi, P. Lievens, S. Ottolenghi, and R. Mantovani
NF-Y Organizes the gamma -Globin CCAAT Boxes Region
J. Biol. Chem., July 3, 1998; 273(27): 16880 - 16889.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. R. Alonso, C. G. Pesce, and A. R. Kornblihtt
The CCAAT-binding Proteins CP1 and NF-I Cooperate with ATF-2 in the Transcription of the Fibronectin Gene
J. Biol. Chem., September 6, 1996; 271(36): 22271 - 22279.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.