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Nucleic Acids Research, 1994, Vol. 22, No. 9 1637-1639
© 1994

GENOME STRUCTURE AND MAPPING

Systematic sequencing of the Escherichia coli genome: analysis of the 2.4–4.1 min (110,917–193,643 bp) region

Nobuyuki Fujita, Hirotada Mori1, Takashi Yura1 and Akira Ishihama*,

Department of Molecular Genetics, National Institute of Genetics Mishima, Shizuoka 411 1Institute for Virus Research, Kyoto University Sakyo-ku, Kyoto 606, Japan

*To whom correspondence should be addressed

Received January 24, 1994. Revised March 28, 1994. Accepted March 28, 1994.

The complete sequence analysis of the E.coli genome was initiated as a collaborative study in Japan. Following the initial analysis of the 0–2.4 min region (Yura, T. et al. (1992) Nucleic Acids Res. 20, 3305–3308), a contiguous sequence of 82,727 bp corresponding to the 2.4–4.1 min region (110,917–193,643 bp as counted from 0 min) was determined. The resulting sequence was found to contain at least 33 known genes and 24 putative genes predicted from protein sequence homology.


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