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Nucleic Acids Research, 1994, Vol. 22, No. 9 1712-1718
© 1994

RNA

A tRNA gene mapping within the chloroplast rDNA cluster is differentially expressed during the development of Daucus carota

F. Manna, D.R. Massardo, K. Wolf1,*, G. Luccarini2, M.S. Carlomagno3, F. Rivellini3, P. Alifano3 and L.Del Giudice

Istituto Internazionale di Genetica e Biofisica, C.N.R. Via G. Marconi 12, I-80125 Napoli, Italy 1Institut für Biologie IV (Mikrobiologie), Rheinisch-Westfälische Technische Hochschule Worringer Weg, D-52056 Aachen, Germany 2Istituto di Mutagenesi e Differenziamento, C.N.R. Via Svezia 10, I-56124 Pisa 3Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle Ricerche, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Via S. Pansini 5, I-80131 Napoli, Italy

*To whom correspondence should be addressed

Received November 2, 1993. Revised March 30, 1994. Accepted March 30, 1994.

In vivo analysis of expression of the chloroplast rDNA cluster during somatic embryogenesis of Daucus carota (D.carota) was performed by Northern-blot analysis with different DNA probes, spanning both the 16S rRNA gene, the 16S–23S rRNA spacer, which contains the two mosaic tRNA genes tRNAlle and tRNAAla, and the region upstream of the 16S rRNA gene, where a tRNAVal maps. We show that expression both of the spacer tRNAs tRNAlle and tRNAAla is not significantly regulated during development whereas the amount of the transcript corresponding to tRNAVal is not detectable during early embryonic stages and progressively accumulates during late phases. Multiple transcription start sites have been identified upstream of the tRNAVal gene by S1 mapping analysis, which are activated late during the embryogenesis. These data indicate that developmental control mechanisms act on plastid gene expression during embryogenesis in carrot.


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