Skip Navigation

This Article
Right arrow Print PDF (505K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gabriel, K.
Right arrow Articles by Rausch, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gabriel, K.
Right arrow Articles by Rausch, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 1 58-63
© 1995

MOLECULAR BIOLOGY

The actinophage RP3 DNA integrates site-specifically into the putative tRNAArg(AGG) gene of Streptomyces rimosus

Karin Gabriel, Holger Schmid, Ute Schmidt and Helmut Rausch*

Institut für Genetik und Mikrobiologie der Universitat München Maria-Ward-Strasse 1A, 80638 München, Germany

*To whom correspondence should be addressed

Received October 10, 1994. Revised November 29, 1994. Accepted November 29, 1994.

The temperate actinophage RP3 integrates site–specifically into the chromosome of Streptomyces rimosus R6-554. The phage attachment site attP and the hybrid attachment sites of the integrated prophage—attL and attR—were cloned and sequenced. The 54nt core sequence, common to all RP3 related attachment sites, comprises the 3' terminal end of a putative tRNAArg(AGG) gene. AttB bears the complete tRNA gene which is restored in attL after integration. A 7.5kb HindIll fragment, bearing attP, was used to construct an integrative plasmid to simulate the integration process in vivo and to localize the phage genes necessary for site specific integration. The mt and xis genes were sequenced and compared to other recombination genes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
Y. Furuta, K. Abe, and I. Kobayashi
Genome comparison and context analysis reveals putative mobile forms of restriction-modification systems and related rearrangements
Nucleic Acids Res., January 12, 2010; (2010) gkp1226v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Muller, H. Shen, D. Hofmann, H. U. Schairer, and J. R. Kirby
Integration into the Phage Attachment Site, attB, Impairs Multicellular Differentiation in Stigmatella aurantiaca
J. Bacteriol., March 1, 2006; 188(5): 1701 - 1709.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
D. C. Alexander, D. J. Devlin, D. D. Hewitt, A. C. Horan, and T. J. Hosted
Development of the Micromonospora carbonacea var. africana ATCC 39149 bacteriophage pMLP1 integrase for site-specific integration in Micromonospora spp.
Microbiology, September 1, 2003; 149(9): 2443 - 2453.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. P. Williams
Integration sites for genetic elements in prokaryotic tRNA and tmRNA genes: sublocation preference of integrase subfamilies
Nucleic Acids Res., February 15, 2002; 30(4): 866 - 875.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Semsey, B. Blaha, K. Koles, L. Orosz, and P. P. Papp
Site-Specific Integrative Elements of Rhizobiophage 16-3 Can Integrate into Proline tRNA (CGG) Genes in Different Bacterial Genera
J. Bacteriol., January 1, 2002; 184(1): 177 - 182.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. Gindreau, R. Lopez, and P. Garcia
MM1, a Temperate Bacteriophage of the Type 23F Spanish/USA Multiresistant Epidemic Clone of Streptococcus pneumoniae: Structural Analysis of the Site-Specific Integration System
J. Virol., September 1, 2000; 74(17): 7803 - 7813.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.