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Nucleic Acids Research, 1995, Vol. 23, No. 10 1671-1678
© 1995

MOLECULAR BIOLOGY

Sequencing of an upstream region of the human HLA-DRA gene containing X' and Y' boxes

Giordans Feriotto1,2, Carlo Mischiati2, Nicoletta Bianchi2, Cristina Rutigliano1, Patrizio Giacomini3 and Roberto Gambari1,2,*

1Centra Interdipartimentale di Biotecnologie Italy 2Dipartimento di Biochimica e Biologia Molecolare Università di Ferrara, 44100 Ferrara, Italy 3Laboratorio di Immunologia, Istituto Regina Elena Roma, Italy

* To whom correspondence should be addressed at: Department of Biochemistry and Molecular Biology, University of Ferrara, Via L. Borsari n. 46, 44100 Ferrara, Italy

Received February 27, 1995. Revised April 6, 1995. Accepted April 6, 1995.

In this paper we report the characterization of a newly sequenced 5' upstream region of the human HLA-DRA gene. We performed (i) search for transcription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (III) search for regions homologous to the 5' upstream sequences of the murine EA gene, (iv) DNase I footprinting experiments and (v) electrophoretic mobility shift assays. Our results demonstrate the existence, in the HLA-DRA gene, of Y' and X' boxes highly homologous to the Y and X boxes present in MHC class II genes, but oriented in the opposite direction. These Y' and X' boxes have been conserved during the molecular evolution of both human HLA-DRA and murine EA genes. DNase I footprinting and gel retardation experiments suggest that the X' and Y' boxes of the HLA-DRA upstream gene region are specifically recognized by nuclear proteins that also bind to the X and Y boxes of the HLA-DRA proximal promoter, respectively.


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