Skip Navigation

This Article
Right arrow Print PDF (5478K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bang, D. d.
Right arrow Articles by Brouwer, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bang, D. d.
Right arrow Articles by Brouwer, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 10 1679-1685
© 1995

RNA

Regulation of the Saccharomyces cerevisiae DNA repair gene RAD16

Dang duong Bang, Vincent Timmermans, Richard Verhage, Anne Marie Zeeman, Pieter van de Putte and Jaap Brouwer*

Laboratory of Molecular Genetics, Department of Biochemistry, Leiden Institute for Chemistry, Goriaeus Laboratories Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands

* To whom correspondence should be addressed

Received February 23, 1995. Revised April 5, 1995. Accepted April 5, 1995.

The RAD16 gene product has been shown to be essential for the repair of the silenced mating type loci [Bang et al. (1992) Nucleic Acids Res. 20, 3925–3931]. More recently we demonstrated that the RAD16 and RAD7 proteins are also required for repair of non-transcribed strands of active genes in Saccharomyces cerevisiae [Waters et al. (1993) Mol. Gen. Genet. 239, 28–32]. We have studied the regulation of the RAD16 gene and found that the RAD16 transcript levels increased up to 7-fold upon UV irradiation. Heat shock at 42°C also results in elevated levels of RAD16mRNA. In sporulatlng MAT{alpha}{alpha}MATa diplold cells RAD16mRNA is also induced. The basal level of the RAD16 transcr1pt is constant during the mltotic cell cycle. G1-arrested cells show normal Induction of RAD16mRNA upon UV irradiation demonstrating that the Induction is not a secondary consequence of G2 cell cycle arrest following UV irradiation. However, in cells arrested in G1 the induction of RAD16 mRNA after UV irradiation is not followed by a rapid decline as occurs In normal growing cells suggesting that the down regulation of RAD16 transcription is dependent on progression into the cell cycle.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
M. U. Fikus, P. A. Mieczkowski, P. Koprowski, J. Rytka, E. ledziewska-Gójska, and Z. Ciela
The Product of the DNA Damage-Inducible Gene of Saccharomyces cerevisiae, DIN7, Specifically Functions in Mitochondria
Genetics, January 1, 2000; 154(1): 73 - 81.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
J. A. Solinger, D. Pascolini, and W.-D. Heyer
Active-Site Mutations in the Xrn1p Exoribonuclease of Saccharomyces cerevisiae Reveal a Specific Role in Meiosis
Mol. Cell. Biol., September 1, 1999; 19(9): 5930 - 5942.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Rodriguez, J. Talamantez, W. Huang, S. H. Reed, Z. Wang, L. Chen, W. J. Feaver, E. C. Friedberg, and A. E. Tomkinson
Affinity Purification and Partial Characterization of a Yeast Multiprotein Complex for Nucleotide Excision Repair Using Histidine-tagged Rad14 Protein
J. Biol. Chem., December 18, 1998; 273(51): 34180 - 34189.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.