Skip Navigation

This Article
Right arrow Print PDF (9556K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (32)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chu, W. M.
Right arrow Articles by Schmid, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chu, W. M.
Right arrow Articles by Schmid, C. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 10 1750-1757
© 1995

MOLECULAR BIOLOGY

RNA polymerase III promoter and terminator elements affect Alu RNA expression

Wen Ming Chu, Wen Man Liu and Carl W. Schmid1,2,*

1Section of Molecular and Cellular Biology Davis, CA 95616, USA 2Department of Chemistry, University of California Davis, CA 95616, USA

* To whom correspondence should be addressed

Received January 27, 1995. Revised April 3, 1995. Accepted April 3, 1995.

Promoter elements derived from the 7SL RNA gene stimulate RNA polymerase III (Pol III) directed Alu transcription In vitro. These elements also stimulate expression of Alus transfected into 293 cells, but transcripts from these same constructs are undetect-able in HeLa cells. A terminator resembling the terminator for the 7SL RNA gene has no effect on In vitro Alu template activity, but increases expression In vivo In a position independent manner. Alu transcripts generated from templates with and without this terminator have identical half-lives, indicating that this terminator stimulates expression by increasing template activity. Together, these results show that Alu expression may be regulated at multiple levels and can respond to cls-acting elements. This new found ability to express Alu transcripts by transient transfectlon provides an opportunity to monitor their post-trans-criptional fate. Primary Alu transcripts are not extensively adenylated or deadenylated following transcription, but are short-lived compared to 118 nt scAlu RNA. In addition to Alu RNA, transfected templates encode scAlu RNA, but very high levels of Alu RNA expression does not increase the abundance of sc Alu RNA. ScAlu RNA is not merely a transient RNA degradation product, but is instead tightly regulated by factors other than the abundance of primary transcripts.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
T. Pelissier, C. Bousquet-Antonelli, L. Lavie, and J.-M. Deragon
Synthesis and processing of tRNA-related SINE transcripts in Arabidopsis thaliana
Nucleic Acids Res., July 28, 2004; 32(13): 3957 - 3966.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
A. M. Roy-Engel, A.-H. Salem, O. O. Oyeniran, L. Deininger, D. J. Hedges, G. E. Kilroy, M. A. Batzer, and P. L. Deininger
Active Alu Element "A-Tails": Size Does Matter
Genome Res., September 1, 2002; 12(9): 1333 - 1344.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Jansa, C. Burek, E. E. Sander, and I. Grummt
The transcript release factor PTRF augments ribosomal gene transcription by facilitating reinitiation of RNA polymerase I
Nucleic Acids Res., January 15, 2001; 29(2): 423 - 429.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Aleman, A. M. Roy-Engel, T. H. Shaikh, and P. L. Deininger
Cis-acting influences on Alu RNA levels
Nucleic Acids Res., December 1, 2000; 28(23): 4755 - 4761.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T.-H. Li, C. Kim, C. M. Rubin, and C. W. Schmid
K562 cells implicate increased chromatin accessibility in Alu transcriptional activation
Nucleic Acids Res., August 15, 2000; 28(16): 3031 - 3039.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Goodier and R. J. Maraia
Terminator-specific Recycling of a B1-Alu Transcription Complex by RNA Polymerase III Is Mediated by the RNA Terminus-binding Protein La
J. Biol. Chem., October 2, 1998; 273(40): 26110 - 26116.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W.-M. Chu, R. Ballard, B. W. Carpick, B. R. G. Williams, and C. W. Schmid
Potential Alu Function: Regulation of the Activity of Double-Stranded RNA-Activated Kinase PKR
Mol. Cell. Biol., January 1, 1998; 18(1): 58 - 68.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
W.-M. Chu, Z. Wang, R. G. Roeder, and C. W. Schmid
RNA Polymerase III Transcription Repressed by Rb through Its Interactions with TFIIIB and TFIIIC2
J. Biol. Chem., June 6, 1997; 272(23): 14755 - 14761.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. W. Englander and B. H. Howard
A Naturally Occurring T(14)A[IMAGE] Tract Blocks Nucleosome Formation Over the Human Neurofibromatosis Type 1 (NF1)-Alu Element
J. Biol. Chem., March 8, 1996; 271(10): 5819 - 5823.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Ueda and G. Chaudhuri
Differential Expression of B1-containing Transcripts in Leishmania-exposed Macrophages
J. Biol. Chem., June 16, 2000; 275(25): 19428 - 19432.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.