Nucleic Acids Research, 1995, Vol. 23, No. 11 1876-1881
© 1995
MOLECULAR BIOLOGY |
Compact structures of d(CNG)n oligonucleotides in solution and their possible relevance to Fragile X and related human genetic diseases
Biophysics Laboratories, University of Portsmouth St Michael's Building, White Swan Road, Portsmouth PO1 2DT, UK
* To vyhom correspondence should be addressed
Received March 10, 1995. Revised April 27, 1995. Accepted April 27, 1995.
We show that oligonucleotides of CNG tracts readily adopt compact DNA structures that move unusually fast on gels. Base composition does not explain this, and non-CNG triplets (including GNC) do not form such structures. Chemical probing and melting experiments suggest that the structures probably are not hairpins. Although both long and short tracts can adopt compact structures, the structure formed by longer tracts is more compact than that formed by shorter ones. We note the possibility that such structures may form In vivo, and be instrumental in normal and/or abnormal function of human genes.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. V. Mirkin and S. M. Mirkin Replication Fork Stalling at Natural Impediments Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 13 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kaushik and S. Kukreti Structural polymorphism exhibited by a quasipalindrome present in the locus control region (LCR) of the human {beta}-globin gene cluster Nucleic Acids Res., July 19, 2006; 34(12): 3511 - 3522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Amrane, B. Sacca, M. Mills, M. Chauhan, H. H. Klump, and J.-L. Mergny Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats Nucleic Acids Res., July 21, 2005; 33(13): 4065 - 4077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pinheiro, G. Scarlett, A. Rodger, P. M. Rodger, A. Murray, T. Brown, S. F. Newbury, and J. A. McClellan Structures of CUG Repeats in RNA. POTENTIAL IMPLICATIONS FOR HUMAN GENETIC DISEASES J. Biol. Chem., September 13, 2002; 277(38): 35183 - 35190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Fojtik and M. Vorlickova The fragile X chromosome (GCC) repeat folds into a DNA tetraplex at neutral pH Nucleic Acids Res., November 15, 2001; 29(22): 4684 - 4690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Deissler, M. Wilm, B. Genc, B. Schmitz, T. Ternes, F. Naumann, M. Mann, and W. Doerfler Rapid Protein Sequencing by Tandem Mass Spectrometry and cDNA Cloning of p20-CGGBP. A NOVEL PROTEIN THAT BINDS TO THE UNSTABLE TRIPLET REPEAT 5'-d(CGG)n-3' IN THE HUMAN FMR1 GENE J. Biol. Chem., July 4, 1997; 272(27): 16761 - 16768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Weitzmann, K. J. Woodford, and K. Usdin DNA Secondary Structures and the Evolution of Hypervariable Tandem Arrays J. Biol. Chem., April 4, 1997; 272(14): 9517 - 9523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ohshima, S. Kang, J. E. Larson, and R. D. Wells Cloning, Characterization, and Properties of Seven Triplet Repeat DNA Sequences J. Biol. Chem., July 12, 1996; 271(28): 16773 - 16783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Deissler, A. Behn-Krappa, and W. Doerfler Purification of Nuclear Proteins from Human HeLa Cells That Bind Specifically to the Unstable Tandem Repeat (CGG)[IMAGE] in the Human FMR1 Gene J. Biol. Chem., February 23, 1996; 271(8): 4327 - 4334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Wells and R. D. Wells Molecular Basis of Genetic Instability of Triplet Repeats J. Biol. Chem., February 9, 1996; 271(6): 2875 - 2878. [Full Text] [PDF]
|
|