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Nucleic Acids Research, 1995, Vol. 23, No. 11 1882-1886
© 1995

MOLECULAR BIOLOGY

Molecular cloning of cDNA encoding the small subunit of Drosophila transcription initiation factor TFIIF

Da-Wei Gong, Mark A. Mortin1, Masami Horikoshi3 and Yoshihiro Nakatani2,*

National Institute of Diabetes and Digestive and Kidney Diseases USA 1 National Cancer Institute USA 2 National Institute of Child Health and Human Development Building 6, Room 416, National Institutes of Health, Bethesda, MD 20892, USA 3 Laboratory of Developmental Biology, Institute of Molecular and Cellular Biosciences University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

* To whom correspondence should be addressed

Received March 7, 1995. Revised April 13, 1995. Accepted April 13, 1995.

Transcription Initiation factor TFIIF is a tetramer consisting of two large subunits (TFllF{alpha} or RAP74) and two small subunits (TFIIFβ or RAP30). We report here the molecular cloning of a Drosophila cDNA encoding TFIIFβ. The cDNA clone contains an open-reading frame encoding a 277 amlno acid polypeptlde having a calculated molecular mass of 32107 Da. Comparison of the deduced amino acid sequence with the corresponding sequences from vertebrates showed only 50% identity, with four insertion/deletion points. For transcription activity in a TFIIF-depleted Drosophila nuclear extract, both TFllF{alpha} and TFIIFβ are essential. Moreover, Drosophila TFIIFp interacts with both Drosophila and human TFllF{alpha} in vitro Thus we conclude that Isolated cDNA encodes bona fide TFIIFβ. The structural domains of TFIIFβ and its sequence similarity to bacterial a factors are discussed.


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