Nucleic Acids Research, 1995, Vol. 23, No. 11 1919-1922
© 1995
MOLECULAR BIOLOGY |
The T-T pyrimidine (6–4) pyrimidinone UV photoproduct is much less mutagenic in yeast than in Escherichia coli
Department of Biophysics, University of Rochester School of Medicine and Dentistry Rochester, NY 14642, USA
* To whom correspondence should be addressed
Received February 21, 1995. Revised April 11, 1995. Accepted April 11, 1995.
We have examined the mutagenic properties of the T-T pyrimidine (6–4) pyrimidinone UV photoproduct In Saccharomyces cerevlslae, transforming the yeast cells either with single-stranded vectors that carried this adduct at a unique site or with gapped duplex vectors In which the adduct was located within a 28 nt single-stranded region. In an earlier study with SOS-induced Escherichia coli we found that this photoproduct Is highly mutagenic, specifically generating 3' T
C substitutions in >85% of replicated molecules, and ascribed this specificity to the formation of a stable guanlne-pyrlmldlnone mispair via hydrogen bonds at N-3 and 0-2. In constrast, this adduct is very much less mutagenic in yeast, with 60–70% of molecules being replicated accurately and only 12–20% of them exhibiting 3' TC substitutions. The enhanced accuracy may reflect the ability of a yeast DNA polymerase, but not E.coll DNA polymerase III, to trap the adduct in a configuration favorable for the formation of an adenine-pyrimidinone base pair.
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