Skip Navigation

This Article
Right arrow Print PDF (5495K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Qin, M.
Right arrow Articles by Ow, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qin, M.
Right arrow Articles by Ow, D. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 11 1923-1927
© 1995

MOLECULAR BIOLOGY

Site-specific cleavage of chromosomes in vitro through Cre-lox recombination

Minmin Qin, Elsa Lee, Todd Zankel and David W. Ow*

Plant Gene Expression Center, US Department of Agriculture 800 Buchanan Street, Albany, CA 94710, USA and Department of Plant Biology, University of California, Berkeley CA 94720, USA

* To whom correspondence should be addressed

Received February 21, 1995. Revised April 18, 1995. Accepted April 18, 1995.

Site-specific recombination systems are useful tools for chromosome engineering In vivo and site-specific DNA cleavage methods have applications in genome analysis and gene isolation. Here, we report a new method to fragment chromosomes In vitro using the Cre-lox site-specific recombination system. Two lox sites were targeted into the 5.7 Mb chromosome I of Schlzosaccharomyces pombe. In vitro recombination between chromosomal lox sites and exogenously provided lox ollgonucleotides ‘cleaved’ the chromosome at the defined lox sequences. Site-specific cleavage of lox sites in the tobacco genome was also demonstrated. This recombination-based cleavage method provides a novel approach for structural and functional analyses of eukaryotic chromosomes as it allows direct isolation of chromosome regions that correspond to phenotypes revealed through Cre-lox mediated chromosome rearrangements in vivo. Moreover, recombination with end-labeled lox ollgonucleotides would permit the specific end-labeling of chromosome segments to facilitate the long range mapping of chromosomes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Choi, D. Begum, H. Koshinsky, D. W. Ow, and R. A. Wing
A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination
Nucleic Acids Res., April 1, 2000; 28(7): e19 - e19.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.