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Nucleic Acids Research, 1995, Vol. 23, No. 11 1928-1935
© 1995

MOLECULAR BIOLOGY

The optimal binding sequence of the Hox11 protein contains a predicted recognition core motif

Shaojun Tang1,2,* and Martin L. Breitman1,2,{dagger}

1 Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Avenue, Toronto, Ontario M5G 1X5, Canada 2 Department of Molecular and Medical Genetics, University of Toronto Toronto, Ontario, Canada

*To whom correspondence should be addressed at present address: Department of Genetics, Research Institute, Room 11-125, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada

Received February 21, 1995. Revised April 24, 1995. Accepted April 24, 1995.

HOX11 is a homeobox-contalnlng oncogene of specific T-cell leukemias. We determined the DNA binding specificity of the Hox11 protein by using a novel technique of random ollgonucleotide selection developed in this study. The optimal Hox11 binding sequence, GGCGGTAAGTGG, contained a core TAAGTG motif that Is consistent with a prediction based on the residues at specific positions that potentially make DNA base contacts and models of homeo-domain-DNA interaction proposed from studies with other homeodomains. The specific interaction between Hox11 and the selected optimal binding sequence was further confirmed by band-shift and DNA competition assays. Given that the Hox11 homeodomain shares low homology with other well studied homeodomains, the presence of a predictable recognition core motif in its optimal binding sequence supports the notion that different homeodomains interact with DNA In a similar manner, through highly conserved residues at specific positions that allow contact with DNA.

{dagger}Deceased


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