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Nucleic Acids Research, 1995, Vol. 23, No. 11 1928-1935
© 1995

MOLECULAR BIOLOGY

The optimal binding sequence of the Hox11 protein contains a predicted recognition core motif

Shaojun Tang1,2,* and Martin L. Breitman1,2,{dagger}

1Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Avenue, Toronto, Ontario M5G 1X5, Canada 2Department of Molecular and Medical Genetics, University of Toronto Toronto, Ontario, Canada

*To whom correspondence should be addressed at present address: Department of Genetics, Research Institute, Room 11-125, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada

Received February 21, 1995. Revised April 24, 1995. Accepted April 24, 1995.

HOX11 is a homeobox-contalnlng oncogene of specific T-cell leukemias. We determined the DNA binding specificity of the Hox11 protein by using a novel technique of random ollgonucleotide selection developed in this study. The optimal Hox11 binding sequence, GGCGGTAAGTGG, contained a core TAAGTG motif that Is consistent with a prediction based on the residues at specific positions that potentially make DNA base contacts and models of homeo-domain-DNA interaction proposed from studies with other homeodomains. The specific interaction between Hox11 and the selected optimal binding sequence was further confirmed by band-shift and DNA competition assays. Given that the Hox11 homeodomain shares low homology with other well studied homeodomains, the presence of a predictable recognition core motif in its optimal binding sequence supports the notion that different homeodomains interact with DNA In a similar manner, through highly conserved residues at specific positions that allow contact with DNA.

{dagger}Deceased


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