Nucleic Acids Research, 1995, Vol. 23, No. 11 1948-1955
© 1995
MOLECULAR BIOLOGY |
Parathyroid hormone/parathyroid hormone related peptide receptor gene transcripts are expressed from tissue-specific and ubiquitous promoters
Department of Physiology, Mclntyre Medical Sciences Building McGill University, 3655 Drummond Street, Montreal, Quebec H3G 1Y6, Canada
* To whom correspondence should be addressed
Received February 10, 1995. Revised April 17, 1995. Accepted April 17, 1995.
Parathyroid hormone (PTH) and PTH related peptide (PTHrP) stimulate diverse physiological responses in a number of tissues by binding to the same receptor. We have previously cloned the gene encoding the mouse PTH/PTHrP receptor (PTHR), and have identified a promoter region. The first exon transcribed from this promoter contains untranslated sequence and is followed by an exon encoding signal sequence and the first amino acids of the mature polypeptide. We have now identified and characterized a second promoter region, located >3 kb upstream of the original. Four partial cDNA clones, amplified from mouse kidney RNA by reverse transcription followed by the poly-merase chain reaction, contain sequence corresponding to two previously unidentified exons composed of untranslated sequence. The second (3') of the two exons is spliced to the previously identified signal sequence exon. These cDNAs are highly homologous to the 5' end of a cDNA Isolated from human kidney, strongly suggesting that the promoter region Is conserved between mouse and humans. RNase protection and primer extension experiments have identified several transcriptional start sites extending over a region of –100 bp. Unlike the previously identified promoter, this promoter is not (G+C)-rich. It lacks a consensus TATA element, but does contain a consensus CCAAT box. We have determined the expression patterns of both promoters by RNase protection with total and poly A+ RNA from several mouse tissues. The newly identified promoter is highly tissue specific, being strongly active In kidney and weakly active in liver, but not expressed in the other tissues studied. The previously Identified (G+C)-rich promoter is expressed In all tissues studied. This indicates that the PTHR gene expression is controlled by regulatory signals specific to kidney and liver, as well as signals functioning in a wide variety of cell types. These results may provide insight into certain defects In PTH signalling found In humans.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. N. Retting, B. Song, B. S. Yoon, and K. M. Lyons BMP canonical Smad signaling through Smad1 and Smad5 is required for endochondral bone formation Development, April 1, 2009; 136(7): 1093 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Kim, G.-S. Lee, E.-M. Jung, K.-C. Choi, G.-T. Oh, and E.-B. Jeung Dexamethasone differentially regulates renal and duodenal calcium-processing genes in calbindin-D9k and -D28k knockout mice Exp Physiol, January 1, 2009; 94(1): 138 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. O. Boylan, L. I. Jepeal, and M. M. Wolfe Sp1/Sp3 binding is associated with cell-specific expression of the glucose-dependent insulinotropic polypeptide receptor gene Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1287 - E1295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Friedman and W. G. Goodman PTH(1-84)/PTH(7-84): a balance of power Am J Physiol Renal Physiol, May 1, 2006; 290(5): F975 - F984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Minagawa, T. Yasuda, T. Watanabe, K. Minamitani, Y. Takahashi, D. Goltzman, J. H. White, G. N. Hendy, and Y. Kohno Association between AAAG Repeat Polymorphism in the P3 Promoter of the Human Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor Gene and Adult Height, Urinary Pyridinoline Excretion, and Promoter Activity J. Clin. Endocrinol. Metab., April 1, 2002; 87(4): 1791 - 1796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Minagawa, T. Watanabe, Y. Kohno, H. Mochizuki, G. N. Hendy, D. Goltzman, J. H. White, and T. Yasuda Analysis of the P3 Promoter of the Human Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor Gene in Pseudohypoparathyroidism Type 1b J. Clin. Endocrinol. Metab., March 1, 2001; 86(3): 1394 - 1397. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Minagawa, M. Y. Kwan, J. D. Bettoun, F. W. Mansour, J. Dassa, G. N. Hendy, D. Goltzman, and J. H. White Dissection of Differentially Regulated (G+C)-Rich Promoters of the Human Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor Gene Endocrinology, July 1, 2000; 141(7): 2410 - 2421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Chikatsu, S. Fukumoto, Y. Takeuchi, M. Suzawa, T. Obara, T. Matsumoto, and T. Fujita Cloning and Characterization of Two Promoters for the Human Calcium-sensing Receptor (CaSR) and Changes of CaSR Expression in Parathyroid Adenomas J. Biol. Chem., March 10, 2000; 275(11): 7553 - 7557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mannstadt, H. Juppner, and T. J. Gardella Receptors for PTH and PTHrP: their biological importance and functional properties Am J Physiol Renal Physiol, November 1, 1999; 277(5): F665 - F675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Rubin, P. Hellman, L. I. Zon, C. J. Lobb, C. Bergwitz, and H. Juppner A G Protein-coupled Receptor from Zebrafish Is Activated by Human Parathyroid Hormone and Not by Human or Teleost Parathyroid Hormone-related Peptide. IMPLICATIONS FOR THE EVOLUTIONARY CONSERVATION OF CALCIUM-REGULATING PEPTIDE HORMONES J. Biol. Chem., August 13, 1999; 274(33): 23035 - 23042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Karperien, H. Farih-Sips, J. A.A. Hendriks, B. Lanske, S. E. Papapoulos, A.-B. Abou-Samra, C. W.G.M. Löwik, and L. H.K. Defize Identification of a Retinoic Acid-Inducible Element in the Murine PTH/PTHrP (Parathyroid Hormone/Parathyroid Hormone-Related Peptide) Receptor Gene Mol. Endocrinol., July 1, 1999; 13(7): 1183 - 1196. [Abstract] [Full Text]
|
|
|
|
|
|
L. Portois, B. Maget, M. Tastenoy, J. Perret, and M. Svoboda Identification of a Glucose Response Element in the Promoter of the Rat Glucagon Receptor Gene J. Biol. Chem., March 19, 1999; 274(12): 8181 - 8190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jagger, A. Gallagher, T. Chambers, and M. Pondel The Porcine Calcitonin Receptor Promoter Directs Expression of a Linked Reporter Gene in a Tissue and Developmental Specific Manner in Transgenic Mice Endocrinology, January 1, 1999; 140(1): 492 - 499. [Abstract] [Full Text]
|
|
|
|
|
|
K. V. Barnes, G. Cheng, M. M. Dawson, and D. R. Menick Cloning of Cardiac, Kidney, and Brain Promoters of the Feline ncx1 Gene J. Biol. Chem., April 25, 1997; 272(17): 11510 - 11517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Bettoun, M. Minagawa, M. Y. Kwan, H. S. Lee, T. Yasuda, G. N. Hendy, D. Goltzman, and J. H. White Cloning and Characterization of the Promoter Regions of the Human Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor Gene: Analysis of Deoxyribonucleic Acid from Normal Subjects and Patients with Pseudohypoparathyroidism Type 1b J. Clin. Endocrinol. Metab., April 1, 1997; 82(4): 1031 - 1040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Joun, B. Lanske, M. Karperien, F. Qian, L. Defize, and A. Abou-Samra Tissue-Specific Transcription Start Sites and Alternative Splicing of the Parathyroid Hormone (PTH)/PTH-Related Peptide (PTHrP) Receptor Gene: A New PTH/PTHrP Receptor Splice Variant that Lacks the Signal Peptide Endocrinology, April 1, 1997; 138(4): 1742 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Amizuka, H. S. Lee, M. Y. Kwan, A. Arazani, H. Warshawsky, G. N. Hendy, H. Ozawa, J. H. White, and D. Goltzman Cell-Specific Expression of the Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor Gene in Kidney from Kidney-Specific and Ubiquitous Promoters Endocrinology, January 1, 1997; 138(1): 469 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Anusaksathien, C. Laplace, X. Li, Y. Ren, L. Peng, S. R. Goldring, and D. L. Galson Tissue-specific and Ubiquitous Promoters Direct the Expression of Alternatively Spliced Transcripts from the Calcitonin Receptor Gene J. Biol. Chem., June 15, 2001; 276(25): 22663 - 22674. [Abstract] [Full Text] [PDF]
|
|