Nucleic Acids Research, 1995, Vol. 23, No. 11 2030-2036
© 1995
MOLECULAR BIOLOGY |
Analysis of the proteins and cis-acting elements regulating the stress-induced phage shock protein operon
Laboratory of Genetics, The Rockefeller University New York, NY 10021, USA 1 Department of Biochemistry and Cell Biology State University of New York, Stony Brook, NY 11794, USA
* To whom correspondence should be addressed
Received January 10, 1995. Revised March 23, 1995. Accepted March 23, 1995.
The phage shock protein operon (pspABCE) of Es-cherichla coll is strongly induced by adverse environmental conditions. Expression is controlled principally at the transcriptlonal level, and transcription is directed by the sigma factor 
54. PspB and PspC are required for high-level psp expression during osmotic shock, ethanol treatment and f1 infection, but heat-Induced expression is Independent of these proteins. We report here that the promoter region contains an upstream activation sequence (UAS) that is required for psp induction and has the enhancer-like ability to activate at a distance. A DNA-bindlng activity is detected in crude protein extracts that Is dependent on the UAS and Induced by heat shock. We further show that Integration host factor (IHF) binds in vitro to a site between the UAS and 54 recognition sequence. In bacteria lacking IHF, psp expression is substantially reduced In response to high temperature and ethanol. During osmotic shock In contrast, psp expression Is only weakly stimulated by IHF, and IHF mutants can strongly induce the operon. The dependence of psp expression on IHF varies with the inducing condition, but does not correlate with dependence on PspB and PspC, indicating distinct, agent-specific activation mechanisms.
+Present addresses: Department of Cell Biology, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA
Department of Dermatology, Harvard Medical School and Cutaneous Biology Research Center, MGH East, 13th Street, Building 149, Charlestown, MA 02129, USA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. M. A. Gronewold and D. Kaiser Mutations of the Act Promoter in Myxococcus xanthus J. Bacteriol., March 1, 2007; 189(5): 1836 - 1844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Aeling, M. L. Opel, N. R. Steffen, V. Tretyachenko-Ladokhina, G. W. Hatfield, R. H. Lathrop, and D. F. Senear Indirect Recognition in Sequence-specific DNA Binding by Escherichia coli Integration Host Factor: THE ROLE OF DNA DEFORMATION ENERGY J. Biol. Chem., December 22, 2006; 281(51): 39236 - 39248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Maxson and A. J. Darwin Multiple promoters control expression of the Yersinia enterocolitica phage-shock-protein A (pspA) operon. Microbiology, April 1, 2006; 152(Pt 4): 1001 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Lloyd, S. E. Jones, G. Jovanovic, P. Gyaneshwar, M. D. Rolfe, A. Thompson, J. C. Hinton, and M. Buck Identification of a New Member of the Phage Shock Protein Response in Escherichia coli, the Phage Shock Protein G (PspG) J. Biol. Chem., December 31, 2004; 279(53): 55707 - 55714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Brown and K. J. Shaw A Novel Family of Escherichia coli Toxin-Antitoxin Gene Pairs J. Bacteriol., November 15, 2003; 185(22): 6600 - 6608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Adams, W. Teertstra, J. Demmers, R. Boesten, and J. Tommassen Interactions between Phage-Shock Proteins in Escherichia coli J. Bacteriol., February 15, 2003; 185(4): 1174 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Reitzer and B. L. Schneider Metabolic Context and Possible Physiological Themes of {sigma}54-Dependent Genes in Escherichia coli Microbiol. Mol. Biol. Rev., September 1, 2001; 65(3): 422 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Mathews and P. Timms Identification and Mapping of Sigma-54 Promoters in Chlamydia trachomatis J. Bacteriol., November 1, 2000; 182(21): 6239 - 6242. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. R. Belitsky and A. L. Sonenshein An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis PNAS, August 31, 1999; 96(18): 10290 - 10295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Mason, J. Dünner, P. Indra, and T. Colangelo Heat-Induced Expression and Chemically Induced Expression of the Escherichia coli Stress Protein HtpG Are Affected by the Growth Environment Appl. Envir. Microbiol., August 1, 1999; 65(8): 3433 - 3440. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Dworkin, A. J. Ninfa, and P. Model A protein-induced DNA bend increases the specificity of a prokaryotic enhancer-binding protein Genes & Dev., March 15, 1998; 12(6): 894 - 900. [Abstract] [Full Text]
|
|