Nucleic Acids Research, 1995, Vol. 23, No. 12 2097-2104
© 1995
MOLECULAR BIOLOGY |
Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1 (MCM3) and CDC46 proteins
Research Center for Molecular Genetics, Hokkaido University Kita 10 Nishi 8, Kita-ku, Sapporo 060, Japan 1Department of Chemistry II, Faculty of Science, Hokkaido University Kita 10 Nishi 8, Kita-ku, Sapporo 060, Japan
* To whom correspondence should be addressed
Received April 12, 1995. Accepted May 2, 1995.
Two new mouse genes encoding proteins that belong to the yeast mlnichromosome maintenance (MCM) protein family, which is involved in the initiation of ONA replication, were isolated and their nucleotlde sequence was determined. They were a putative CDC46/MCM5 homolog and a putative cdc21 homolog. About 30% amlno acid identity was obtained between members In the family, and >40% between the putative mouse and yeast homologs. The expression of these genes was cell-cycle specific at the late G1 to S phase. Immunochemical analyses showed the physical interaction between mouse P1MCM3 and CDC46 protein. These results suggest that MCM proteins function in co-ordination for DNA replication.
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