Nucleic Acids Research, 1995, Vol. 23, No. 12 2137-2146
© 1995
MOLECULAR BIOLOGY |
clRF-3, a new member of the interferon regulatory factor (IRF) family that is rapidly and transiently induced by dsRNA
Cancer Research Laboratories, Queen's University Kingston, Ontario K7L 3N6, Canada
* To whom correspondence should be addressed
Received February 15, 1995. Revised April 24, 1995. Accepted April 24, 1995.
In mammals, some of the effects of interferon (IFN) on gene transcription are known to be mediated by a family of IFN-inducible DNA-blnding proteins, the IFN regulatory factor (IRF) family, which includes both activators and repressors of transcription. Although IFN activities have been described in many vertebrates, little Is known about regulation of IFN- or IFN-stimulated genes in species other than human and mouse. Here, we report the cloning of a chicken cDNA, clRF-3, encoding a protein wfth a DNA-binding domain similar to that found in the mammalian IRF family of proteins. Similarity between clRF-3 and the mammalian IRFs is comparable with that between known members of the family. It is most similar to the IRF proteins ICSBP and ISGF3
but is equally divergent from both. Gel mobility shift assays indicate that clRF-3 is capable of binding a known IFN-stimulated response element that Is conserved between the mammalian and chicken Mx genes. Expression of the clRF-3 gene can be induced to high levels by poly(l)*poly(C). Induction is rapid and transient with no requirement for protein synthesis. Co-treatment of cells with cycloheximide results in superinduction of clRF-3 mRNA. The structural and regulatory characteristics of clRF-3 indicate that it is the first example of a non-mammalian IRF protein.
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