Skip Navigation

This Article
Right arrow Print PDF (5452K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tuusa, J.
Right arrow Articles by Syvaoja, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tuusa, J.
Right arrow Articles by Syvaoja, J. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 12 2178-2183
© 1995

MOLECULAR BIOLOGY

Human DNA polymerase {varepsilon} is expressed during cell proliferation in a manner characteristic of replicative DNA polymerases

Jussi Tuusa, Lahja Uitto and Juhani E. Syvaoja*

Biocenter Oulu and Department of Biochemistry, University of Oulu FIN-90570 Oulu, Finland

* To whom correspondence should be addressed

Received February 28, 1995. Revised May 1, 1995. Accepted May 1, 1995.

In order to shed light on the role of mammalian DNA polymerase {varepsilon} we studied the expression of mRNA for the human enzyme during cell proliferation and during the cell cycle. Steady-state levels of mRNA encoding DNA polymerase {varepsilon} were elevated dramatically when quiescent (G0) cells were stimulated to proliferate (G1/S) in a similar manner to those of DNA polymerase {alpha} Message levels of DNA polymerase ß were unchanged In similar experiments. The concentration of Immunoreactlve DNA polymerase e was also much higher in extracts from proliferating tissues than In those from non-proliferating or slowly proliferating tissues. The level of DNA polymerase {varepsilon} mRNA in actively cycling cells synchronized with nocodazole and in cells fractionated by counterflow centrifugal elutrfation showed weaker variation, being at its highest at the G1/S stage boundary. The results presented strongly suggest that mammalian DNA polymerase {varepsilon} is involved In the replication of chromosomal DNA and/or in a repair process that may be substantially activated during the replication of chromosomal DNA. A hypothetical role for DNA polymerase {varepsilon} in a repair process coupled to replication Is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. Matsumoto, K. Yaginuma, A. Igarashi, M. Imura, M. Hasegawa, K. Iwabuchi, T. Date, T. Mori, K. Ishizaki, K. Yamashita, et al.
Perturbed gap-filling synthesis in nucleotide excision repair causes histone H2AX phosphorylation in human quiescent cells
J. Cell Sci., March 15, 2007; 120(6): 1104 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Vens, E. Dahmen-Mooren, M. Verwijs-Janssen, W. Blyweert, L. Graversen, H. Bartelink, and A. C. Begg
The role of DNA polymerase {beta} in determining sensitivity to ionizing radiation in human tumor cells
Nucleic Acids Res., July 1, 2002; 30(13): 2995 - 3004.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Fuss and S. Linn
Human DNA Polymerase epsilon Colocalizes with Proliferating Cell Nuclear Antigen and DNA Replication Late, but Not Early, in S Phase
J. Biol. Chem., March 1, 2002; 277(10): 8658 - 8666.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A.-L. Ducrest, M. Amacker, Y. D. Mathieu, A. P. Cuthbert, D. A. Trott, R. F. Newbold, M. Nabholz, and J. Lingner
Regulation of Human Telomerase Activity: Repression by Normal Chromosome 3 Abolishes Nuclear Telomerase Reverse Transcriptase Transcripts but Does Not Affect c-Myc Activity
Cancer Res., October 1, 2001; 61(20): 7594 - 7602.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Huang, M. Jokela, J. Tuusa, S. Skog, K. Poikonen, and J. E. Syvaoja
E2F mediates induction of the Sp1-controlled promoter of the human DNA polymerase {{epsilon}} B-subunit gene POLE2
Nucleic Acids Res., July 1, 2001; 29(13): 2810 - 2821.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
W. Liu and S. Linn
Proteolysis of the human DNA polymerase {varepsilon} catalytic subunit by caspase-3 and calpain specifically during apoptosis
Nucleic Acids Res., November 1, 2000; 28(21): 4180 - 4188.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Yamada, C. Masutani, S. Iwai, and F. Hanaoka
Complementation of defective translesion synthesis and UV light sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase {eta}
Nucleic Acids Res., July 1, 2000; 28(13): 2473 - 2480.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Maki, K. Hashimoto, T. Ohara, and A. Sugino
DNA Polymerase II (epsilon ) of Saccharomyces cerevisiae Dissociates from the DNA Template by Sensing Single-stranded DNA
J. Biol. Chem., August 14, 1998; 273(33): 21332 - 21341.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Makiniemi, T. Hillukkala, J. Tuusa, K. Reini, M. Vaara, D. Huang, H. Pospiech, I. Majuri, T. Westerling, T. P. Makela, et al.
BRCT Domain-containing Protein TopBP1 Functions in DNA Replication and Damage Response
J. Biol. Chem., August 3, 2001; 276(32): 30399 - 30406.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.