Nucleic Acids Research, 1995, Vol. 23, No. 12 2305-2313
© 1995
MOLECULAR BIOLOGY |
Characterization of a glucocorticoid responsive element and identification of an AT-rich element that regulate the link protein gene
The Laboratory of Developmental Biology, National Institute of Dental Research NIH, Bethesda, MD 20892-4370, USA
* To whom correspondence should be addressed
Received January 3, 1995. Revised May 8, 1995. Accepted May 8, 1995.
The cartilage matrix Is composed of characteristic components Including type II collagen, aggrecan and link protein. In this paper, we report two DNA elements that regulate the link protein gene. Using transient transfection assays with link protein gene constructs In chondrocytes, chloramphenlcol acetyl transferase (CAT) assays were used to measure the transcriptional activity of the link protein gene. Previously, we Identified an enhancer-like activity within the first Intron of the gene. In this paper, we report an active 34 bp (+1390 to +1424) fragment within this region that contains a glucocorticoid-like response element (GRE). Both deletion of, and site-specific mutations within this sequence motif reduced the dexamethasone-lnductbte activity. The GRE-iike sequence from the rat link protein gene, or the homologous sequence from the human link protein gene were Included in vectors containing the thymldine kinase promoter linked to the CAT gene (tkCAT). Both human and rat elements transferred the ability to respond to dexamethasone and hydrocortJ-sone with a >10-fold induction. Deletions through the promoter from –923 to –900 Identified a second site required for both glucocorticoid and serum responsiveness. A four base substitution at this site resulted In a loss of serum responsiveness. This region contains an AT-rich element, similar to the AT-rich elements involved in homeotic protein regulation of the growth hormone gene and the muscle creatine kinase gene. Southwestern analysis using oligonucleotides containing the AT-rich element from the link protein gene or the muscle creatine kinase gene, identified a 32 kDa protein band from nuclear extracts of chick chondrocytes. Using these AT-rich ollgonudeotides In band-shift analyses, nuclear extracts of chick sternal muscle, rat chondrosarcoma and chick sternal chondrocytes each showed formation of different complexes suggesting cell specificity. AT-rich elements have been identified as binding sites for homeodomain-containing proteins and can contribute to gene regulation by serum response factors. The Identification of an AT-rich element in the link protein gene suggests similar functions for this element.
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