Skip Navigation

This Article
Right arrow Print PDF (3236K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sagawa, H.
Right arrow Articles by Kato, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sagawa, H.
Right arrow Articles by Kato, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 13 2367-2370
© 1995

ENZYMOLOGY

Sse83871, a useful eight cutter for mammalian genopme analysis (influence of methylation on the activity of Sse83871)

Hiroaki Sagawa*, Atsushi Ohshima and Ikunoshin Kato

Genetic Engineering Section II, Biotechnology Research Laboratories Takara Shuzo Co.Ltd, seta 3-4-1,Otu

*To whom corespondence should be addressed

Received April 21, 1995. Accepted May 17, 1995.

To develop restriction enzymes that are useful for genome analysis, we previously performed screening and isolated Sse8387l from Streptomyces sp. strain 8387. Sse63871 is a restriction enzyme that recognizes 5-CCTGCA/GG-3' and cleaves DNA at the site shown by the diagonal (Nucleic Acid Res., 18, 5637-5640). The present study evaluated the effects of methylation that is Important when Sse8387l is used for genome analysis. Sse6387l lost cleavage activity after methylation of adenine or methylation of cytosine at any site in the recognition sequence. However, the recognition sequence of Sse6387l contains no CG sequence, which Is the mammalian methylation sequence. In addition, we evaluated the effects of methylation of CG at sites other than the recognition sequence. The cleavage activity of Sse83871 was maintained even when CG sequences were present immediately before or after, or near the recognition sequence, and cytosine was methylated. These results suggest that CG methylation does not affect the cleavage activity of Sse6387l. Therefore, Sse8387l seems to be very useful for mammalian genome analysis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.