Nucleic Acids Research, 1995, Vol. 23, No. 13 2464-2471
© 1995
ENZYMOLOGY |
Zinc(II) ions selectively interact with DNA sequences present at the TFIIIA binding site of the Xenopus 5S-RNA gene
Department de Biologia Molecular i celluar, Centre d'Investigacio Desenvolupament-CSIC Jordi Girona Salgoda 18-26, 08034 Barcelona, Spain
*To whom corespondence should be addressed
Received April 1, 1995. Accepted May 30, 1995.
It has been known for some time that zinc, as well as most transition metal ions, is capable of binding to the DNA bases. However, little is known about the presence and distribution of metal binding sites along naturally occurring genomic DNA molecules. In this paper, the interaction of zinc with the Xenopus 5S-RNA gene has been studied and several metal binding sites have been Identified on the basis of the changes inchemical reactivity observed in the presence of the metal. The strongest zJnc-bindlng sites of the Xenopus 5S-RNA gene correspond to GGG trinucleotlde repeats. Some GG dinucleotides also show a significant affinity for zinc. Interestingly, the binding site for TFMIA, a zJnc-flnger transcription factor, contains several sites with strong zinc affinity. In particular, a TGGGA sequence which Is essential for the binding of TFIIIA shows the strongest affinity for zinc. The conformational properties of this DNA sequence, together with the high electronegative potential of GGG runs, Is likely to determine its strong affinity for zinc. The possible biological relevance of these results is discussed.
+Present address:Laborotory of Biochemistry, National Caner Institue, NIH Bethedsa, MA 20892, USA
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