Nucleic Acids Research, 1995, Vol. 23, No. 14 2593-2599
© 1995
MOLECULAR BIOLOGY |
Activation of mammalian ribosomal gene transcription requires phosphorylation of the nucleolar transcription requires phosphorylation of the nucleolar trasnscription factor UBF
Division of Molecular Biology of the Cell II, German Cancer Research Center D-69120 Heidelberg, Germany
*To whom correspondence should be addressed
Received May 9, 1995. Accepted June 14, 1995.
The nucleolar factor UBF Is phosphorylated by casein kinase II (CKII) at serlne residues within the C-terminal acidic domain which is required for transcriptionactivation. To investigate the biological significance of UBF modification, we have compared the trans-activating properties of cellular UBF and recombinant UBF expressed in Escherichla coll. Using a variety of assays we demonstrate that unphosphorylated UBF is transcriptionally Inactive and has to be phosphorylated at multiple sites to stimulate transcription. Examination of cDNA mutants in which the serine residues within the C-terminal domain were altered by site-directed mutagenesis demonstrates that CKIImedlated phosphorylatlons of UBF contribute to, but are not sufficient for, transcriptlonal activation. Besides CKII, other cellular protein kinases phosphorylate UBF at distinct sites in a growth-dependent manner. The marked differences in the tryptic peptide maps of UBF from growing and serum-starved cells suggest that alterations in the degree of UBF phosphorylation may modulate rRNA synthetic activity in response to extracellular signals.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. H. Lin, M. D. Platt, S. B. Ficarro, M. H. Hoofnagle, J. Shabanowitz, L. Comai, D. F. Hunt, and G. K. Owens Mass spectrometric identification of phosphorylation sites of rRNA transcription factor upstream binding factor Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1617 - C1624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Lin, S. Navarro, S. Reddy, and L. Comai CK2-mediated stimulation of Pol I transcription by stabilization of UBF-SL1 interaction Nucleic Acids Res., October 18, 2006; 34(17): 4752 - 4766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Panova, K. I. Panov, J. Russell, and J. C. B. M. Zomerdijk Casein kinase 2 associates with initiation-competent RNA polymerase I and has multiple roles in ribosomal DNA transcription. Mol. Cell. Biol., August 1, 2006; 26(16): 5957 - 5968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Lawrence, B. McStay, and D. A. Matthews Nucleolar protein upstream binding factor is sequestered into adenovirus DNA replication centres during infection without affecting RNA polymerase I location or ablating rRNA synthesis J. Cell Sci., June 15, 2006; 119(12): 2621 - 2631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Sheng, Y. Liang, C.-Y. Lin, L. Comai, and W. J. Chirico Direct Regulation of rRNA Transcription by Fibroblast Growth Factor 2 Mol. Cell. Biol., November 1, 2005; 25(21): 9419 - 9426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, A. Wu, H. Sun, R. Drakas, C. Garofalo, S. Cascio, E. Surmacz, and R. Baserga Functional Significance of Type 1 Insulin-like Growth Factor-mediated Nuclear Translocation of the Insulin Receptor Substrate-1 and {beta}-Catenin J. Biol. Chem., August 19, 2005; 280(33): 29912 - 29920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wu, X. Tu, M. Prisco, and R. Baserga Regulation of Upstream Binding Factor 1 Activity by Insulin-like Growth Factor I Receptor Signaling J. Biol. Chem., January 28, 2005; 280(4): 2863 - 2872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sun and R. Baserga Deletion of the Pleckstrin and Phosphotyrosine Binding Domains of Insulin Receptor Substrate-2 Does Not Impair Its Ability to Regulate Cell Proliferation in Myeloid Cells Endocrinology, November 1, 2004; 145(11): 5332 - 5343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Cui, T. Elsam, Q. Tian, J. T. Seykora, M. Grachtchouk, A. Dlugosz, and H. Tseng Gli Proteins Up-Regulate the Expression of Basonuclin in Basal Cell Carcinoma Cancer Res., August 15, 2004; 64(16): 5651 - 5658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Valdez, D. Henning, R. B. So, J. Dixon, and M. J. Dixon The Treacher Collins syndrome (TCOF1) gene product is involved in ribosomal DNA gene transcription by interacting with upstream binding factor PNAS, July 20, 2004; 101(29): 10709 - 10714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Drakas, X. Tu, and R. Baserga Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase PNAS, June 22, 2004; 101(25): 9272 - 9276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Prisco, A. Maiorana, C. Guerzoni, G. Calin, B. Calabretta, R. Voit, I. Grummt, and R. Baserga Role of Pescadillo and Upstream Binding Factor in the Proliferation and Differentiation of Murine Myeloid Cells Mol. Cell. Biol., June 15, 2004; 24(12): 5421 - 5433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Bjerregaard, C. Wrenzycki, V. V. Philimonenko, P. Hozak, J. Laurincik, H. Niemann, J. Motlik, and P. Maddox-Hyttel Regulation of Ribosomal RNA Synthesis During the Final Phases of Porcine Oocyte Growth Biol Reprod, April 1, 2004; 70(4): 925 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hannan, Y. Brandenburger, A. Jenkins, K. Sharkey, A. Cavanaugh, L. Rothblum, T. Moss, G. Poortinga, G. A. McArthur, R. B. Pearson, et al. mTOR-Dependent Regulation of Ribosomal Gene Transcription Requires S6K1 and Is Mediated by Phosphorylation of the Carboxy-Terminal Activation Domain of the Nucleolar Transcription Factor UBF{dagger} Mol. Cell. Biol., December 1, 2003; 23(23): 8862 - 8877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Grummt Life on a planet of its own: regulation of RNA polymerase I transcription in the nucleolus Genes & Dev., July 15, 2003; 17(14): 1691 - 1702. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sun, X. Tu, M. Prisco, A. Wu, I. Casiburi, and R. Baserga Insulin-Like Growth Factor I Receptor Signaling and Nuclear Translocation of Insulin Receptor Substrates 1 and 2 Mol. Endocrinol., March 1, 2003; 17(3): 472 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Cavanaugh, I. Hirschler-Laszkiewicz, Q. Hu, M. Dundr, T. Smink, T. Misteli, and L. I. Rothblum Rrn3 Phosphorylation Is a Regulatory Checkpoint for Ribosome Biogenesis J. Biol. Chem., July 19, 2002; 277(30): 27423 - 27432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Karmakar, J. Piotrowski, R. M. Brosh Jr., J. A. Sommers, S. P. L. Miller, W.-H. Cheng, C. M. Snowden, D. A. Ramsden, and V. A. Bohr Werner Protein Is a Target of DNA-dependent Protein Kinase in Vivo and in Vitro, and Its Catalytic Activities Are Regulated by Phosphorylation J. Biol. Chem., May 17, 2002; 277(21): 18291 - 18302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. HUANG, T. WU, L. XU, A. LIU, Y. JI, and G. HU Upstream binding factor up-regulated in hepatocellular carcinoma is related to the survival and cisplatin-sensitivity of cancer cells FASEB J, March 1, 2002; 16(3): 293 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. O'Sullivan, G. J. Sullivan, and B. McStay UBF Binding In Vivo Is Not Restricted to Regulatory Sequences within the Vertebrate Ribosomal DNA Repeat Mol. Cell. Biol., January 15, 2002; 22(2): 657 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Voit and I. Grummt Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription PNAS, October 31, 2001; (2001) 231071698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q Tian, G. Kopf, R. Brown, and H Tseng Function of basonuclin in increasing transcription of the ribosomal RNA genes during mouse oogenesis Development, January 2, 2001; 128(3): 407 - 416. [Abstract] [PDF]
|
|
|
|
 |
|
 Y.-G. Ko, Y.-S. Kang, E.-K. Kim, S. G. Park, and S. Kim Nucleolar Localization of Human Methionyl-tRNA Synthetase and Its Role in Ribosomal RNA Synthesis J. Cell Biol., May 1, 2000; 149(3): 567 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Paule and R. J. White SURVEY AND SUMMARY Transcription by RNA polymerases I and III Nucleic Acids Res., March 15, 2000; 28(6): 1283 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Comai, Y. Song, C. Tan, and T. Bui Inhibition of RNA Polymerase I Transcription in Differentiated Myeloid Leukemia Cells by Inactivation of Selectivity Factor 1 Cell Growth Differ., January 1, 2000; 11(1): 63 - 70. [Abstract] [Full Text]
|
|
|
|
|
|
H.-K. Chen, C.-Y. Pai, J.-Y. Huang, and N.-H. Yeh Human Nopp140, Which Interacts with RNA Polymerase I: Implications for rRNA Gene Transcription and Nucleolar Structural Organization Mol. Cell. Biol., December 1, 1999; 19(12): 8536 - 8546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Klein and I. Grummt Cell cycle-dependent regulation of RNA polymerase I transcription: The nucleolar transcription factor UBF is inactive in mitosis and early G1 PNAS, May 25, 1999; 96(11): 6096 - 6101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhai and L. Comai A Kinase Activity Associated with Simian Virus 40 Large T Antigen Phosphorylates Upstream Binding Factor (UBF) and Promotes Formation of a Stable Initiation Complex between UBF and SL1 Mol. Cell. Biol., April 1, 1999; 19(4): 2791 - 2802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Solow, L. Lezina, and P. M. Lieberman Phosphorylation of TFIIA Stimulates TATA Binding Protein-TATA Interaction and Contributes to Maximal Transcription and Viability in Yeast Mol. Cell. Biol., April 1, 1999; 19(4): 2846 - 2852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Tuan, W. Zhai, and L. Comai Recruitment of TATA-Binding Protein-TAFI Complex SL1 to the Human Ribosomal DNA Promoter Is Mediated by the Carboxy-Terminal Activation Domain of Upstream Binding Factor (UBF) and Is Regulated by UBF Phosphorylation Mol. Cell. Biol., April 1, 1999; 19(4): 2872 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-C. Albert, M. Denton, M. Kermekchiev, and C. S. Pikaard Histone Acetyltransferase and Protein Kinase Activities Copurify with a Putative Xenopus RNA Polymerase I Holoenzyme Self-Sufficient for Promoter-Dependent Transcription Mol. Cell. Biol., January 1, 1999; 19(1): 796 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Kihm, J. C. Hershey, T. A. J. Haystead, C. S. Madsen, and G. K. Owens Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein PNAS, December 8, 1998; 95(25): 14816 - 14820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Trifa, I. Privat, J. Gagnon, L. Baeza, and S. Lerbs-Mache The Nuclear RPL4 Gene Encodes a Chloroplast Protein That Co-purifies with the T7-like Transcription Complex as Well as Plastid Ribosomes J. Biol. Chem., February 13, 1998; 273(7): 3980 - 3985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Voit and I. Grummt Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription PNAS, November 20, 2001; 98(24): 13631 - 13636. [Abstract] [Full Text] [PDF]
|
|