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Nucleic Acids Research, 1995, Vol. 23, No. 14 2600-2607
© 1995

MOLECULAR BIOLOGY

Novel DNA binding protiens highly specific to UV-damaged DNA sequences from embryos of Drosophila melanogaster

Mihoko Kai, Toshiaki Takahashi, Takeshi Todo1 and Kengo Sakaguchi*

Department of Applied Biological Science,Faculty of Science and Technology, Science University of Tokyo Noda-shi, Chiba-ken 278, Japan 1The Department of Radiation biology,Faculty of Medicine, Osaka University Yamadaoka 2-2, Suita, Osaka 565, Japan

*To whom correspondence should be addressed

Received May 5, 1995. Accepted June 15, 1995.

Three new proteins which selectively bind to UVdamaged DNA were identified and purified to near homogeneity from UV-lrradiated Drosophila melanogaster embryos through several column chromatographles. These proteins, tentatively designated as D-DDB P1, P2 and P3, can be identified as different complex bands in a gel shift assay by using UVirradiated TC-31 probe DNA. Analysis of the purified D-DDB P1 fraction by native or SDS-polyacrylamlde gel electrophoresis and FPLC-Superose 6 gel filtration demonstrated that it is a monomer protein which Is a 30 kDa polypeptlde. The D-DDB P2 protein is a monopolypeptide with a molecular mass of 14 kDa. Both D-DDB P1 and P2 highly prefer binding to UV-irradiated DNA, and have almost no affinity for non-irradiated DNA. Gel shift assays with either UV-irradiated DNA probes demonstrated that D-DDB P1 may show a preference for binding to (6–4) photoproducts, while D-DDB P2 may prefer binding to pyrimidine dimers. Both these proteins require magnesium ions for binding. D-DDB P1 is an ATP-preferent protein. These findings are discussed in relation to two recently described [Todo and Ryo (1991) Mutat. Res., 273, 85–93; Todo et al. (1993) Nature, 361, 371–374] DNA-binding factors from Drosophila cell extracts. A possible role for these DNA-binding proteins in lesion recognition and DNA repair of UV-induced photoproducts is discussed.


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K.-i. Takata, G. Ishikawa, F. Hirose, and K. Sakaguchi
Drosophila damage-specific DNA-binding protein 1 (D-DDB1) is controlled by the DRE/DREF system
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