Nucleic Acids Research, 1995, Vol. 23, No. 14 2626-2628
© 1995
MOLECULAR BIOLOGY |
Trans-activation and DNA-binding properties of the transcription factor, Sox-18
Centre for Molecular and Cellular Biology, University of Queensland St Lucia, 4072 Queensland, Australia
*To whom correspondence should be addressed
Received April 25, 1995. Accepted June 14, 1995.
Sox-18 is a member of the Sox multi-gene family (Sry-related HMG-box gene). We have bacterially expressed this 378 amlno acid protein and demonstrated sequence-specific binding to the Sox DNA-binding motif AACAAAG. A distinct 95 amino acid activation domain was mapped in Sox-18 using GAL4-Sox-18 fusions (amino acids 160–225). Furthermore, Sox-18 was capable of trans-actlvatlng gene expression through the AACAAAG motif. Our results suggest that Sox-18 functions as a classical frans-activator of gene expression.
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