Nucleic Acids Research, 1995, Vol. 23, No. 14 2734-2741
© 1995
MOLECULAR BIOLOGY |
USF binds to the APB
sequence in the promoter of the amyloid ß-protein precursor gene
Department of Psychiatry and Behavioral Science, State University of New York at Stony Brook Stony Brook,NY 11794–8101, USA 1Unité 273 de I'INSERM, Université de Nice-Sophia Antipolis 06034 Nice, France
Received March 16, 1995. Accepted May 31, 1995.
The APB
domain in the amyloid ß-protein precursor (APP) promoter contains a nuclear factor binding domain with the core recognition sequence TCAGCTGAC. Proteins in nuclear extracts from brain and numerous cell lines bind to this domain and it contributes –10–30% to the basal APP promoter activity. Included in this domain is the CANNTG motif, which is recognized by basic helix-loop-helix transcription factors. The same motif is also present in the CDEI element of the yeast centromere and in the adenovirus major late promoter (AdMLP). Here we present evidence based on thermostabilrty, relative binding affinity, electrophoretic mobility and antibody recognition that the cellular proteins that bind to the APB and CDEI motifs are USF. However, the relative binding affinity for the motifs is different. The affinity of USF for AdMLP is 
20–fold higher than for the APBa sequence and 5–fold higher than for the CDEI sequence. Mutational analysis suggested that the primary determinant for USF binding affinity resides within the octamer CAGCTGAC, which is composed of the E-box consensus sequence CANNTG followed by the dinucleotide AC. The human homolog of the mouse CDEI binding protein did not bind to either the CDEI sequence or APB.
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