Skip Navigation

This Article
Right arrow Print PDF (12527K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Aidinis, V.
Right arrow Articles by Guialis, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aidinis, V.
Right arrow Articles by Guialis, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 14 2742-2753
© 1995

RNA

Two immunologically related polypeptides of 72/74 kDa specify a novel 70–11 OS heterogeneous nuclear RNP

Vassillis Aidinis, Constantine E. Sekeris and Apostolia Guialis*

Institute of Biological Research and Biotechnology, The National Hellenic Research Foundation 48 Vas. Constantinou Avenue, Athens 11635, Greece

*To whom correspondence should be addressed

Received March 14, 1995. Accepted June 5, 1995.

Evidence suggesting the presence in rat liver nuclear extracts of a new RNP complex of 70–11 OS has been provided [Hatzoglou, M., Adamtziki, E., Margaritls, L. and Sekeris, C.E (1985) Exp. Cell. Res. 157, 227–241]. Biochemical features unique to this RNP were its stability to salt and mild RNase digestion and the presence of a pair of polypeptides of 72/74 kDa. By producing antibodies against the 72/74 kDa polypeptides these proteins have been defined as Integral components of the 70–11 OS RNP complex. They comprise two Immunologically related polypeptides with an exclusively nucleoplasmic localization, giving a speckled pattern in a diffuse background, similar, but not identical, to the Sm antigen. The 70–11 OS RNP complex, referred to as large heterogeneous nuclear RNP (LH-nRNP), has a simple protein pattern that includes, In addition to the 72/74 kDa proteins, three stably associated polypeptides of apparent molecular size 110,61 and 59 kDa. The bulk of its RNA component represents a discrete RNA population of 10–20S,belonging to a subset of the RNA detected within immunopurified HeLa hnRNP complexes. These RNA species are RNA polymerase II transcripts of greater stability relative to the bulk of hnRNA, containing oligo(A) or poly(A) sequences. Immunodepletlon and/ or antibody addition studies in HeLa splicing extracts using antibodies with specificity for the 72/74 kDa proteins revealed a rather strong inhibition of splicing activity, suggesting participation of the LH-nRNP complex in In vitro splicing.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.