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Nucleic Acids Research, 1995, Vol. 23, No. 14 2770-2774
© 1995

MOLECULAR BIOLOGY

Identification of human autoantibodies to transcription factor IIB

Francena D. Abendroth, Scott R. Peterson, Matthew Galman, Akira Suwa1,+, John A. Hardin1 and William S. Dynan*

Department of Chemistry and Biochemistry, Campus Box 215, University of Colorado Boulder, CO 80309, USA 1Department of Medicine, Medical College of Georgia 1120 15th Street, Augusta, GA 30912, USA

*To whom correspondence should be addressed

Received March 3, 1995. Accepted June 13, 1995.

We have characterized the ability of various human autoimmune sera to react with RNA polymerase II transcription factors. One serum, which strongly Inhibited transcription in a cell-free system, was shown to contain antibodies directed against human TFIIB. The serum did not show reactivity against the other general transcription factors, including human TBP, TRIE and TF1IF. The inhibition of transcription was directly attributable to depletion of TFIIB activity, as demonstrated by reconstftution of activity with recombinant TFIIB. It has long been recognized that components of the RNA processing machinery are major human autoantigens. The present results show that at least one general transcription factor required for messenger RNA synthesis Is an autoantigen as well.

+Present address: Division of Rheumatic Diseases, Tokyo Metropolitan Ohtsuka Hospital, Tokyo 170, Japan


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