Nucleic Acids Research, 1995, Vol. 23, No. 15 2893-2899
© 1995
MOLECULAR BIOLOGY |
8-Hydroxyadenine (7, 8-dihydro-8-Oxoadenine) induces misincorporation in in vitro DNA synthesis and mutations in NIH 3T3 cells
Faculty of Pharmaceutial Sciences, Hokkaido University Kita-, Nishi-6, Kita-Ku, Sapporo 060, Japan 1Cellular Physiology Laboratory, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama 351-01, Japan 2Banyu Tsukuba Research Institute Okubo-3, Tsukuba 300-33, Japan
*To Whom correspondence should be addressed
Received April 28, 1995. Accepted June 16, 1995.
An oligodeoxyribonucleotide containing 8-hydroxyadenine (OH8Ade) was chemically synthesized and single- and double-stranded c-Ha-ras gene fragments with OH8Ade at the second position of codon 61 were prepared. The single-stranded ras gene fragment was used as a template for in vitro DNA synthesis with the Klenow fragment of Escherichla coli DNA polymerase I, Taq DNA polymerase, rat DNA polymerase ß and mouse DNA polymerase 
. The former two enzymes exclusively incorporated dTMP opposite 0H8Ade. The DNA polymerases a and p misinserted dGMP, and dAMP and dGMP, respectively. The c-Ha-ras gene was constructed using the double-stranded ras gene fragment containing OH8Ade and was transfected into NIH 3T3 cells. The gene with OH8Ade induced focus formation, Indicating that OH8Ade elicited point mutations in cells. When c-Ha-ras genes present in transformed cells were analyzed, an A
G transition and an AC transversion were detected. These results indicate that OH8Ade induced misincorporation in in vitro DNA synthesis and mutations in mammalian cells.
+Present address: Department of Environmental Oncology, Institute of Industiral Ecological, Sciences, University of Occupational and Environmental Health, I- Iseigaoka, Kitakyushu 807, Japan
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. R. Trzeciak, S. G. Nyaga, P. Jaruga, A. Lohani, M. Dizdaroglu, and M. K. Evans Cellular repair of oxidatively induced DNA base lesions is defective in prostate cancer cell lines, PC-3 and DU-145 Carcinogenesis, August 1, 2004; 25(8): 1359 - 1370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Jensen, G. Calvayrac, B. Karahalil, V. A. Bohr, and T. Stevnsner Mammalian 8-Oxoguanine DNA Glycosylase 1 Incises 8-Oxoadenine Opposite Cytosine in Nuclei and Mitochondria, while a Different Glycosylase Incises 8-Oxoadenine Opposite Guanine in Nuclei J. Biol. Chem., May 23, 2003; 278(21): 19541 - 19548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. TUO, P. JARUGA, H. RODRIGUEZ, V. A. BOHR, and M. DIZDAROGLU Primary fibroblasts of Cockayne syndrome patients are defective in cellular repair of 8-hydroxyguanine and 8-hydroxyadenine resulting from oxidative stress FASEB J, April 1, 2003; 17(6): 668 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kamiya Mutagenic potentials of damaged nucleic acids produced by reactive oxygen/nitrogen species: approaches using synthetic oligonucleotides and nucleotides: SURVEY AND SUMMARY Nucleic Acids Res., January 15, 2003; 31(2): 517 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tuo, P. Jaruga, H. Rodriguez, M. Dizdaroglu, and V. A. Bohr The Cockayne Syndrome Group B Gene Product Is Involved in Cellular Repair of 8-Hydroxyadenine in DNA J. Biol. Chem., August 16, 2002; 277(34): 30832 - 30837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Malins, P. M. Johnson, T. M. Wheeler, E. A. Barker, N. L. Polissar, and M. A. Vinson Age-related Radical-induced DNA Damage Is Linked to Prostate Cancer Cancer Res., August 1, 2001; 61(16): 6025 - 6028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Malins, N. L. Polissar, G. K. Ostrander, and M. A. Vinson Single 8-oxo-guanine and 8-oxo-adenine lesions induce marked changes in the backbone structure of a 25-base DNA strand PNAS, October 26, 2000; (2000) 230438797. [Abstract] [Full Text]
|
|
|
|
|
|
M. Sabourin and N. Osheroff Sensitivity of human type II topoisomerases to DNA damage: stimulation of enzyme-mediated DNA cleavage by abasic, oxidized and alkylated lesions Nucleic Acids Res., May 1, 2000; 28(9): 1947 - 1954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Tan, A. P. Grollman, and S. Shibutani Comparison of the mutagenic properties of 8-oxo-7,8-dihydro-2'-deoxyadenosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine DNA lesions in mammalian cells Carcinogenesis, December 1, 1999; 20(12): 2287 - 2292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Olinski, P. Jaruga, M. Foksinski, K. Bialkowski, and J. Tujakowski Epirubicin-Induced Oxidative DNA Damage and Evidence for Its Repair in Lymphocytes of Cancer Patients Who Are Undergoing Chemotherapy Mol. Pharmacol., November 1, 1997; 52(5): 882 - 885. [Abstract] [Full Text]
|
|
|
|
|
|
D. C. Malins, N. L. Polissar, and S. J. Gunselman Infrared spectral models demonstrate that exposure to environmental chemicals leads to new forms of DNA PNAS, April 15, 1997; 94(8): 3611 - 3615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Malins, N. L. Polissar, and S. J. Gunselman Models of DNA structure achieve almost perfect discrimination between normal prostate, benign prostatic hyperplasia (BPH), and adenocarcinoma and have a high potential for predicting BPH and prostate cancer PNAS, January 7, 1997; 94(1): 259 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Malins, N. L. Polissar, and S. J. Gunselman Tumor progression to the metastatic state involves structural modifications in DNA markedly different from those associated with primary tumor formation PNAS, November 26, 1996; 93(24): 14047 - 14052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. O. Zharkov, T. A. Rosenquist, S. E. Gerchman, and A. P. Grollman Substrate Specificity and Reaction Mechanism of Murine 8-Oxoguanine-DNA Glycosylase J. Biol. Chem., September 8, 2000; 275(37): 28607 - 28617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Malins, N. L. Polissar, G. K. Ostrander, and M. A. Vinson Single 8-oxo-guanine and 8-oxo-adenine lesions induce marked changes in the backbone structure of a 25-base DNA strand PNAS, November 7, 2000; 97(23): 12442 - 12445. [Abstract] [Full Text] [PDF]
|
|