Nucleic Acids Research, 1995, Vol. 23, No. 15 2919-2928
© 1995
MOLECULAR BIOLOGY |
A novel IL-4 responsive element of the E
MHC class II promoter that binds to an inducible factor
1Institute of Molecular Bioloy and Biotechnology, Foundation for Research and Technology PO Box 1527, Hearklion 711 10, Crete, Greece 2University of Crete, Department of Biology, Heraklion Crete, Greece
*To Whom correspondence should be addressed at: Institute of Molecular Biology and Technology, PO Box 1527, Heraklion 711 10, Crete, Greece
Received April 20, 1995. Accepted June 21, 1995.
lnterleukin-4 (IL-4) Is a lymphokine with important role in the growth and differentiation of T and B lymphocytes. In the latter, IL-4 Induces transcriptionally MHC class II gene expression. Using the M12 mouse lymphoid cell line, we have determined an IL-4 response sequence (ILRS) in the proximal promoter region of the Ea class II gene. The ILRS extends from -80 to -111 and Includes the MHC class IIX motif and 19 bp of additional 5' sequence. In mouse lymphoid cells, IL-4 activates a complex (Nuclear Factor-IL-4, NFIL-4), that binds to a novel element within the ILRS. Similar IL-4 Inducible complexes bind to the Interferon-
response element of the Fey receptor (GRR), the acute phase response element (APRE) of the 
2 macroglobulin promoters and an INFp promoter site, overlapping the PRDII/INFKB element The factor contacts all these elements through their common GGAA motif. NFIL-4 is immunologically unrelated to NFKB or STAT1 proteins that also recognize the above elements. Activation of NFIL-4 requires tyrosine phosphorylation, occurs within 2 mln and persists as long as IL-4 Is present. NFIL-4 has an apparent molecular weight of 75 kDa as determined by sedimentation through glycerol gradients.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  V. T. Thieu, E. T. Nguyen, B. P. McCarthy, H. A. Bruns, R. Kapur, C.-H. Chang, and M. H. Kaplan IL-4-stimulated NF-{kappa}B activity is required for Stat6 DNA binding J. Leukoc. Biol., August 1, 2007; 82(2): 370 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Braunstein, S. Brutsaert, R. Olson, and C. Schindler STATs Dimerize in the Absence of Phosphorylation J. Biol. Chem., September 5, 2003; 278(36): 34133 - 34140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Davey, G. Greicius, J. Thyberg, and E. Severinson STAT6 is required for the regulation of IL-4-induced cytoskeletal events in B cells Int. Immunol., July 1, 2000; 12(7): 995 - 1003. [Abstract] [Full Text] [PDF]
|
|