Skip Navigation

This Article
Right arrow Print PDF (6865K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by O'Prey, J.
Right arrow Articles by Harrison, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Prey, J.
Right arrow Articles by Harrison, P. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 18 3664-3672
© 1995

MOLECULAR BIOLOGY

Tissue-specific regulation of the rabbit 15-lipoxygenase gene in erythroid cells by a transcriptional silencer

Jim O'Prey and Paul R. Harrison*

Beatson Institute for Cancer Research, Cancer Research Campaign Laboratories Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK

*To whom correspondence should be addressed

Received July 3, 1995. Revised August 18, 1995. Accepted August 18, 1995.

The 15-lipoxygenase (lox) gene is expressed in a tissue-specific manner, predominantly in erythroid cells but also in airway epithelial cells and eoslnophils. We demonstrate in this report that the 5' flanking DNA of the 15-lox gene contains sequences which down-regulate its activity in a variety of non-erythrold cell lines but not in two erythroid cell lines. The element has characteristics of a transcriptional ‘silencer’ since it functions in both orientations. The main activity of the silencer has been mapped to the first 900 bp of 5' flanking DNA, which contains nine binding sites for a nuclear factor present in non-erythroid cells but not in erythroid cells. These binding sites have similar sequences and multiple copies of the binding sites confer tissue-specific down-regulation when attached to a minimal lox promoter fragment. The 5' flanking DNA also contains a cluster of three binding sites for the GATA family of transcription factors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
M. A. Trujillo, M. Sakagashira, and N. L. Eberhardt
The Human Growth Hormone Gene Contains a Silencer Embedded within an Alu Repeat in the 3'-Flanking Region
Mol. Endocrinol., October 1, 2006; 20(10): 2559 - 2575.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Gupta, V. Sueblinvong, J. Raman, V. Jeevanandam, and M. P. Gupta
Single-stranded DNA-binding Proteins PUR{alpha} and PUR{beta} Bind to a Purine-rich Negative Regulatory Element of the {alpha}-Myosin Heavy Chain Gene and Control Transcriptional and Translational Regulation of the Gene Expression: IMPLICATIONS IN THE REPRESSION OF {alpha}-MYOSIN HEAVY CHAIN DURING HEART FAILURE
J. Biol. Chem., November 7, 2003; 278(45): 44935 - 44948.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
M. A. Giembycz and M. A. Lindsay
Pharmacology of the Eosinophil
Pharmacol. Rev., June 1, 1999; 51(2): 213 - 340.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Heydeck, L. Thomas, K. Schnurr, F. Trebus, W. E. Thierfelder, J. N. Ihle, and H. Kuhn
Interleukin-4 and -13 Induce Upregulation of the Murine Macrophage 12/15-Lipoxygenase Activity: Evidence for the Involvement of Transcription Factor STAT6
Blood, October 1, 1998; 92(7): 2503 - 2510.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. S. Malone, S. A. Omori, and R. Wall
Silencer elements controlling the B29 (Igbeta ) promoter are neither promoter- nor cell-type-specific
PNAS, November 11, 1997; 94(23): 12314 - 12319.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.