Nucleic Acids Research, 1995, Vol. 23, No. 19 3816-3821
© 1995
MOLECULAR BIOLOGY |
Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase
1Laboaory of Molecular Genetics University of Tokyo 4-6-1 Shirokanedi, Minato-ku, Tokyo 108, Japan 2Deparment of Virology, institute of Medical Science, University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan 3Discovery Research Laboratories III, Sumitomo Pharmaceuticals Research Center 3-1-98 Kasugade-naka, Konohana-ku, Osaka 554, Japan
*To whom correspondence should be addressed
Received August 8, 1995. Accepted August 23, 1995.
A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian colls, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ-expression unit can be activated by the Cre-mediated exclsionai deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene expression was detected in nearly 100% of cultured CV1, HeLa and Jurkat cells. These results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for estabilshing a powerful on/off switching strategy of gene expression incultured mammalian cells and presumably in transgenic animals. The method is also applicable to construction of recombinant Ad bearing a gene the expression of which is deleterious to propagation of recombinant Ad.
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M. Tanaka, H. Kagawa, Y. Yamanashi, T. Sata, and Y. Kawaguchi Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo J. Virol., December 20, 2002; 77(2): 1382 - 1391. [Abstract] [Full Text] [PDF]
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 M. Tsuru, H. Katagiri, T. Asano, T. Yamada, S. Ohno, T. Ogihara, and Y. Oka Role of PKC isoforms in glucose transport in 3T3-L1 adipocytes: insignificance of atypical PKC Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E338 - E345. [Abstract] [Full Text] [PDF]
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Y. Tamori, J. Masugi, N. Nishino, and M. Kasuga Role of Peroxisome Proliferator-Activated Receptor-{gamma} in Maintenance of the Characteristics of Mature 3T3-L1 Adipocytes Diabetes, July 1, 2002; 51(7): 2045 - 2055. [Abstract] [Full Text] [PDF]
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M. D. Johnson, X. Wu, N. Aithmitti, and R. S. Morrison Peg3/Pw1 Is a Mediator between p53 and Bax in DNA Damage-induced Neuronal Death J. Biol. Chem., June 14, 2002; 277(25): 23000 - 23007. [Abstract] [Full Text] [PDF]
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Y. F. Jin, T. Ishibashi, A. Nomoto, and M. Masuda Isolation and Analysis of Retroviral Integration Targets by Solo Long Terminal Repeat Inverse PCR J. Virol., May 3, 2002; 76(11): 5540 - 5547. [Abstract] [Full Text] [PDF]
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S. Sato, M. Nakamura, D. H. Cho, S. J. Tapscott, H. Ozaki, and K. Kawakami Identification of transcriptional targets for Six5: implication for the pathogenesis of myotonic dystrophy type 1 Hum. Mol. Genet., May 1, 2002; 11(9): 1045 - 1058. [Abstract] [Full Text] [PDF]
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 M Akimoto, T Miyahara, J Arai, A Akimoto, H Hamada, Y Yoshida, and N Yoshimura A new delivery system for 5-fluorouracil using prodrug and converting enzyme Br J Ophthalmol, May 1, 2002; 86(5): 581 - 586. [Abstract] [Full Text] [PDF]
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 K. Katagiri, M. Hattori, N. Minato, and T. Kinashi Rap1 Functions as a Key Regulator of T-Cell and Antigen-Presenting Cell Interactions and Modulates T-Cell Responses Mol. Cell. Biol., February 15, 2002; 22(4): 1001 - 1015. [Abstract] [Full Text] [PDF]
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H. Tang, Q. Hao, T. Fitzgerald, T. Sasaki, E. J. Landon, and T. Inagami Pyk2/CAKbeta Tyrosine Kinase Activity-mediated Angiogenesis of Pulmonary Vascular Endothelial Cells J. Biol. Chem., February 8, 2002; 277(7): 5441 - 5447. [Abstract] [Full Text] [PDF]
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N. Takahashi, T. Nemoto, R. Kimura, A. Tachikawa, A. Miwa, H. Okado, Y. Miyashita, M. Iino, T. Kadowaki, and H. Kasai Two-Photon Excitation Imaging of Pancreatic Islets With Various Fluorescent Probes Diabetes, February 1, 2002; 51(90001): S25 - 28. [Abstract] [Full Text] [PDF]
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S. Okabe, T. Urushido, D. Konno, H. Okado, and K. Sobue Rapid Redistribution of the Postsynaptic Density Protein PSD-Zip45 (Homer 1c) and Its Differential Regulation by NMDA Receptors and Calcium Channels J. Neurosci., December 15, 2001; 21(24): 9561 - 9571. [Abstract] [Full Text] [PDF]
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M. Senoo, N. Mochida, L. Wang, Y. Matsumura, D. Suzuki, N. Takeda, Y. Shinkai, and S. Habu Limited effect of chromatin remodeling on D{beta}-to-J{beta} recombination in CD4+CD8+ thymocyte: implications for a new aspect in the regulation of TCR {beta} gene recombination Int. Immunol., November 1, 2001; 13(11): 1405 - 1414. [Abstract] [Full Text] [PDF]
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 T. YOKOO, T. OHASHI, Y. UTSUNOMIYA, H. SHIBA, J. S. SHEN, Y. HISADA, Y. ETO, T. KAWAMURA, and T. HOSOYA Inflamed Glomeruli--Specific Gene Activation that Uses Recombinant Adenovirus with the Cre/loxP System J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2330 - 2337. [Abstract] [Full Text] [PDF]
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Y. Liu, Z. Cseresnyes, W. R. Randall, and M. F. Schneider Activity-dependent nuclear translocation and intranuclear distribution of NFATc in adult skeletal muscle fibers J. Cell Biol., October 1, 2001; 155(1): 27 - 40. [Abstract] [Full Text] [PDF]
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S. Nishimura, M. Adachi, T. Ishida, T. Matsunaga, H. Uchida, H. Hamada, and K. Imai Adenovirus-mediated Transfection of Caspase-8 Augments Anoikis and Inhibits Peritoneal Dissemination of Human Gastric Carcinoma Cells Cancer Res., October 1, 2001; 61(19): 7009 - 7014. [Abstract] [Full Text] [PDF]
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S. Okabe, A. Miwa, and H. Okado Spine Formation and Correlated Assembly of Presynaptic and Postsynaptic Molecules J. Neurosci., August 15, 2001; 21(16): 6105 - 6114. [Abstract] [Full Text] [PDF]
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K. Ueda, M. Iwahashi, M. Nakamori, M. Nakamura, I. Matsuura, H. Yamaue, and H. Tanimura Carcinoembryonic Antigen-specific Suicide Gene Therapy of Cytosine Deaminase/5-Fluorocytosine Enhanced by the Cre/loxP System in the Orthotopic Gastric Carcinoma Model Cancer Res., August 1, 2001; 61(16): 6158 - 6162. [Abstract] [Full Text] [PDF]
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 T. Yokoo, T. Ohashi, Y. Utsunomiya, J. S. Shen, Y. Hisada, Y. Eto, T. Kawamura, and T. Hosoya Genetically modified bone marrow continuously supplies anti-inflammatory cells and suppresses renal injury in mouse Goodpasture syndrome Blood, July 1, 2001; 98(1): 57 - 64. [Abstract] [Full Text] [PDF]
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Y. Nonomura, H. Kohsaka, K. Nasu, Y. Terada, M.-a. Ikeda, and N. Miyasaka Suppression of arthritis by forced expression of cyclin-dependent kinase inhibitor p21Cip1 gene into the joints Int. Immunol., June 1, 2001; 13(6): 723 - 731. [Abstract] [Full Text] [PDF]
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 S. Hanada, Y. Terada, S. Inoshita, S. Sasaki, S. M. Lohmann, A. Smolenski, and F. Marumo Overexpression of protein kinase G using adenovirus inhibits cyclin E transcription and mesangial cell cycle Am J Physiol Renal Physiol, May 1, 2001; 280(5): F851 - F859. [Abstract] [Full Text] [PDF]
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M. Nakano, K. Odaka, M. Ishimura, S. Kondo, N. Tachikawa, J. Chiba, Y. Kanegae, and I. Saito Efficient gene activation in cultured mammalian cells mediated by FLP recombinase-expressing recombinant adenovirus Nucleic Acids Res., April 1, 2001; 29(7): e40 - e40. [Abstract] [Full Text] [PDF]
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K. Kuwahara, Y. Saito, E. Ogawa, N. Takahashi, Y. Nakagawa, Y. Naruse, M. Harada, I. Hamanaka, T. Izumi, Y. Miyamoto, et al. The Neuron-Restrictive Silencer Element-Neuron-Restrictive Silencer Factor System Regulates Basal and Endothelin 1-Inducible Atrial Natriuretic Peptide Gene Expression in Ventricular Myocytes Mol. Cell. Biol., March 15, 2001; 21(6): 2085 - 2097. [Abstract] [Full Text]
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T.-H. Cheng, C.-R. Chang, P. Joy, S. Yablok, and M. R. Gartenberg Controlling gene expression in yeast by inducible site-specific recombination Nucleic Acids Res., December 15, 2000; 28(24): e108 - e108. [Abstract] [Full Text] [PDF]
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K. Nasu, H. Kohsaka, Y. Nonomura, Y. Terada, H. Ito, K. Hirokawa, and N. Miyasaka Adenoviral Transfer of Cyclin-Dependent Kinase Inhibitor Genes Suppresses Collagen-Induced Arthritis in Mice J. Immunol., December 15, 2000; 165(12): 7246 - 7252. [Abstract] [Full Text] [PDF]
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W. Satoh, Y. Hirai, K. Tamayose, and T. Shimada Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination J. Virol., November 15, 2000; 74(22): 10631 - 10638. [Abstract] [Full Text]
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Y. Nagayama, H. Yokoi, K. Takeda, M. Hasegawa, E. Nishihara, H. Namba, S. Yamashita, and M. Niwa Adenovirus-Mediated Tumor Suppressor p53 Gene Therapy for Anaplastic Thyroid Carcinoma in Vitro and in Vivo J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4081 - 4086. [Abstract] [Full Text]
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 H. Obara, A. Takayanagi, J. Hirahashi, K. Tanaka, G. Wakabayashi, K. Matsumoto, M. Shimazu, N. Shimizu, and M. Kitajima Overexpression of Truncated I{kappa}B{alpha} Induces TNF-{alpha}-Dependent Apoptosis in Human Vascular Smooth Muscle Cells Arterioscler Thromb Vasc Biol, October 1, 2000; 20(10): 2198 - 2204. [Abstract] [Full Text] [PDF]
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K. Namikawa, M. Honma, K. Abe, M. Takeda, K. Mansur, T. Obata, A. Miwa, H. Okado, and H. Kiyama Akt/Protein Kinase B Prevents Injury-Induced Motoneuron Death and Accelerates Axonal Regeneration J. Neurosci., April 15, 2000; 20(8): 2875 - 2886. [Abstract] [Full Text] [PDF]
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Y. Banzai, H. Miki, H. Yamaguchi, and T. Takenawa Essential Role of Neural Wiskott-Aldrich Syndrome Protein in Neurite Extension in PC12 Cells and Rat Hippocampal Primary Culture Cells J. Biol. Chem., April 14, 2000; 275(16): 11987 - 11992. [Abstract] [Full Text] [PDF]
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M. Kawanishi, Y. Tamori, H. Okazawa, S. Araki, H. Shinoda, and M. Kasuga Role of SNAP23 in Insulin-induced Translocation of GLUT4 in 3T3-L1 Adipocytes. MEDIATION OF COMPLEX FORMATION BETWEEN SYNTAXIN4 AND VAMP2 J. Biol. Chem., March 10, 2000; 275(11): 8240 - 8247. [Abstract] [Full Text] [PDF]
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 N. Kobayashi, T. Fujiwara, K. A. Westerman, Y. Inoue, M. Sakaguchi, H. Noguchi, M. Miyazaki, J. Cai, N. Tanaka, I. J. Fox, et al. Prevention of Acute Liver Failure in Rats with Reversibly Immortalized Human Hepatocytes Science, February 18, 2000; 287(5456): 1258 - 1262. [Abstract] [Full Text]
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T. Nakanishi, T. Nishida, T. Shimo, K. Kobayashi, T. Kubo, T. Tamatani, K. Tezuka, and M. Takigawa Effects of CTGF/Hcs24, a Product of a Hypertrophic Chondrocyte-Specific Gene, on the Proliferation and Differentiation of Chondrocytes in Culture Endocrinology, January 1, 2000; 141(1): 264 - 273. [Abstract] [Full Text] [PDF]
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 Y. Shintani, H. Yotsuyanagi, K. Moriya, H. Fujie, T. Tsutsumi, Y. Kanegae, S. Kimura, I. Saito, and K. Koike Induction of apoptosis after switch-on of the hepatitis B virus X gene mediated by the Cre/loxP recombination system J. Gen. Virol., December 1, 1999; 80(12): 3257 - 3265. [Abstract] [Full Text]
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A. Asai, J.-h. Qiu, Y. Narita, S. Chi, N. Saito, N. Shinoura, H. Hamada, Y. Kuchino, and T. Kirino High Level Calcineurin Activity Predisposes Neuronal Cells to Apoptosis J. Biol. Chem., November 26, 1999; 274(48): 34450 - 34458. [Abstract] [Full Text] [PDF]
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H. Kuwahara, N. Araki, K. Makino, N. Masuko, S. Honda, K. Kaibuchi, K. Fukunaga, E. Miyamoto, M. Ogawa, and H. Saya A Novel NE-dlg/SAP102-associated Protein, p51-nedasin, Related to the Amidohydrolase Superfamily, Interferes with the Association between NE-dlg/SAP102 and N-Methyl-D-aspartate Receptor J. Biol. Chem., November 5, 1999; 274(45): 32204 - 32214. [Abstract] [Full Text] [PDF]
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