Skip Navigation

This Article
Right arrow Print PDF (1403K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (57)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nemeth, A.
Right arrow Articles by Wahle, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nemeth, A.
Right arrow Articles by Wahle, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 20 4034-4041
© 1995

MOLECULAR BIOLOGY

Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II

Annette Nemeth1,+, Sabine Krause1, Diana Blank1, Andreas Jenny1, Paul Jenö2, Ariel Lustig3 and Elmar Wahle1,*,+

1Department of cell Biology, Universitat Basel Klingelbergstrasse 70, CH-4056 Basel, Switzerland 2Department of Biochemistry, Universitat Basel Klingelbergstrasse 70, CH-4056 Basel, Switzerland 3Department of Biophysics, Biozentrum Universitat Basel Klingelbergstrasse 70, CH-4056 Basel, Switzerland

*To whom correspondence should be addressed

Received July 28, 1995. Revised September 14, 1995. Accepted September 14, 1995.

cDNA clones for bovine poly(A) binding protein II (PAB II) were isolated. Their sequence predicts a protein of 32.8 kDa, revising earlier estimates of molecular mass. The protein contains one putative RNA-binding domain of the RNP type, an acidic N-terminal and a basic C-terminal domain. Analyses of authentic PAB II were in good agreement with all predictions from the cDNA sequence except that a number of arginine residues appeared to be post-translationally modified. Poly(A) binding protein II expressed in Escherichia coli was active in poly(A) binding and reconstitution of processive polyadenylation, including poly(A) tail length control. The cDNA clones showed a number of potential PAB II binding sites in the 3' untranslated sequence. Bovine poly(A)+ RNA contained two mRNAs hybridizing to a PAB II-specific probe. Analysis of a genomic clone revealed six introns in the coding sequence. The revised molecular mass led to a demonstration of PAB II oligomer formation and a reinterpretation of earlier data concerning the protein's binding to poly(A).

+present address: Universität Giessen, Institut für Biochemie. Heinrich-Buff-Ring 58, D-35392 Giessen. Germany


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
U. Kuhn, M. Gundel, A. Knoth, Y. Kerwitz, S. Rudel, and E. Wahle
Poly(A) Tail Length Is Controlled by the Nuclear Poly(A)-binding Protein Regulating the Interaction between Poly(A) Polymerase and the Cleavage and Polyadenylation Specificity Factor
J. Biol. Chem., August 21, 2009; 284(34): 22803 - 22814.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kolbel, C. Ihling, K. Bellmann-Sickert, I. Neundorf, A. G. Beck-Sickinger, A. Sinz, U. Kuhn, and E. Wahle
Type I Arginine Methyltransferases PRMT1 and PRMT-3 Act Distributively
J. Biol. Chem., March 27, 2009; 284(13): 8274 - 8282.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Fronz, S. Otto, K. Kolbel, U. Kuhn, H. Friedrich, A. Schierhorn, A. G. Beck-Sickinger, A. Ostareck-Lederer, and E. Wahle
Promiscuous Modification of the Nuclear Poly(A)-binding Protein by Multiple Protein-arginine Methyltransferases Does Not Affect the Aggregation Behavior
J. Biol. Chem., July 18, 2008; 283(29): 20408 - 20420.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Perreault, C. Lemieux, and F. Bachand
Regulation of the Nuclear Poly(A)-binding Protein by Arginine Methylation in Fission Yeast
J. Biol. Chem., March 9, 2007; 282(10): 7552 - 7562.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Hosoda, F. Lejeune, and L. E. Maquat
Evidence that Poly(A) Binding Protein C1 Binds Nuclear Pre-mRNA Poly(A) Tails
Mol. Cell. Biol., April 15, 2006; 26(8): 3085 - 3097.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. P. TAVANEZ, P. CALADO, J. BRAGA, M. LAFARGA, and M. CARMO-FONSECA
In vivo aggregation properties of the nuclear poly(A)-binding protein PABPN1
RNA, May 1, 2005; 11(5): 752 - 762.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. Kuhn, A. Nemeth, S. Meyer, and E. Wahle
The RNA Binding Domains of the Nuclear poly(A)-binding Protein
J. Biol. Chem., May 2, 2003; 278(19): 16916 - 16925.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Calapez, H. M. Pereira, A. Calado, J. Braga, J. Rino, C. Carvalho, J. P. Tavanez, E. Wahle, A. C. Rosa, and M. Carmo-Fonseca
The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive
J. Cell Biol., December 9, 2002; 159(5): 795 - 805.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. P. Bao, L. J. Cook, D. O'Donovan, E. Uyama, and D. C. Rubinsztein
Mammalian, Yeast, Bacterial, and Chemical Chaperones Reduce Aggregate Formation and Death in a Cell Model of Oculopharyngeal Muscular Dystrophy
J. Biol. Chem., March 29, 2002; 277(14): 12263 - 12269.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
X. Fan, P. Dion, J. Laganiere, B. Brais, and G. A. Rouleau
Oligomerization of polyalanine expanded PABPN1 facilitates nuclear protein aggregation that is associated with cell death
Hum. Mol. Genet., October 1, 2001; 10(21): 2341 - 2351.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
Y.-J. Kim, S. Noguchi, Y. K. Hayashi, T. Tsukahara, T. Shimizu, and K. Arahata
The product of an oculopharyngeal muscular dystrophy gene, poly(A)-binding protein 2, interacts with SKIP and stimulates muscle-specific gene expression
Hum. Mol. Genet., May 1, 2001; 10(11): 1129 - 1139.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Calado, F. M.S. Tome, B. Brais, G.A. Rouleau, U. Kuhn, E. Wahle, and M. Carmo-Fonseca
Nuclear inclusions in oculopharyngeal muscular dystrophy consist of poly(A) binding protein 2 aggregates which sequester poly(A) RNA
Hum. Mol. Genet., September 1, 2000; 9(15): 2321 - 2328.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A Calado and M Carmo-Fonseca
Localization of poly(A)-binding protein 2 (PABP2) in nuclear speckles is independent of import into the nucleus and requires binding to poly(A) RNA
J. Cell Sci., January 6, 2000; 113(12): 2309 - 2318.
[Abstract] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
J. Zhao, L. Hyman, and C. Moore
Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis
Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 405 - 445.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Smith, K. P. Rucknagel, A. Schierhorn, J. Tang, A. Nemeth, M. Linder, H. R. Herschman, and E. Wahle
Unusual Sites of Arginine Methylation in Poly(A)-binding Protein II and in Vitro Methylation by Protein Arginine Methyltransferases PRMT1 and PRMT3
J. Biol. Chem., May 7, 1999; 274(19): 13229 - 13234.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. Kessler, J. Zhao, and C. L. Moore
Purification of the Saccharomyces cerevisiae Cleavage/Polyadenylation Factor I. SEPARATION INTO TWO COMPONENTS THAT ARE REQUIRED FOR BOTH CLEAVAGE AND POLYADENYLATION OF mRNA 3prime ENDS
J. Biol. Chem., October 25, 1996; 271(43): 27167 - 27175.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Lellek, R. Kirsten, I. Diehl, F. Apostel, F. Buck, and J. Greeve
Purification and Molecular Cloning of a Novel Essential Component of the Apolipoprotein B mRNA Editing Enzyme-Complex
J. Biol. Chem., June 23, 2000; 275(26): 19848 - 19856.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Winstall, M. Sadowski, U. Kuhn, E. Wahle, and A. B. Sachs
The Saccharomyces cerevisiae RNA-binding Protein Rbp29 Functions in Cytoplasmic mRNA Metabolism
J. Biol. Chem., July 14, 2000; 275(29): 21817 - 21826.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.