Skip Navigation

This Article
Right arrow Print PDF (1190K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yonaha, M.
Right arrow Articles by Yasukochi, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yonaha, M.
Right arrow Articles by Yasukochi, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 20 4050-4054
© 1995

ENZYMOLOGY

Cell cycle-dependent regulation of RNA polymerase II basal transcription activity

Masatomo Yonaha*, Taku Chibazakura, Shigetaka Kitajima1 and Yukio Yasukochi

Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University 1-5-45 Yushima, Bunkyo-ku, Tokyo 113, Japan 1Department of Clinical and Laboratory Medicine, Kyushu University School of Medicine 3-1-1 Maidashi, Fukuoka 812, Japan

*To whom correspondence should be addressed

Received July 19, 1995. Revised September 18, 1995. Accepted September 18, 1995.

Regulation of transcription by RNA polymerase II (pol II) in eukaryotic cells requires both basal and regulatory transcription factors. In this report we have investigated in vitro pol II basal transcription activity during the cell cycle by using nuclear extracts from synchronized HeLa cells. It is shown that pol II basal transcription activity is low in the S and G2 phases and high in early G1 phase and TFIID is the rate limiting component of pol II basal transcription activity during the cell cycle. Further analyses reveal that TFIID exists as a less active form in the S and G2 phases and nuclear extracts from S and G2 phase cells contain a heat-sensitive repressor(s) of TATA box binding protein (TBP). These results suggest that pol II basal transcription activity is regulated by a qualitative change in the TFIID complex, which could involve repression of TBP, during the cell cycle.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
J. S. Sims, K. T. Militello, P. A. Sims, V. P. Patel, J. M. Kasper, and D. F. Wirth
Patterns of Gene-Specific and Total Transcriptional Activity during the Plasmodium falciparum Intraerythrocytic Developmental Cycle
Eukaryot. Cell, March 1, 2009; 8(3): 327 - 338.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S Vee, L Lafanechere, D Fisher, J Wehland, D Job, and A Picard
Evidence for a role of the (alpha)-tubulin C terminus in the regulation of cyclin B synthesis in developing oocytes
J. Cell Sci., January 3, 2001; 114(5): 887 - 898.
[Abstract] [PDF]

Home page
 J. Virol.Home page
F. Kashanchi, E. T. Agbottah, C. A. Pise-Masison, R. Mahieux, J. Duvall, A. Kumar, and J. N. Brady
Cell Cycle-Regulated Transcription by the Human Immunodeficiency Virus Type 1 Tat Transactivator
J. Virol., January 1, 2000; 74(2): 652 - 660.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.