Nucleic Acids Research, 1995, Vol. 23, No. 20 4081-4086
© 1995
RNA |
Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes
Department of Cell Biology, Harvard Medical School Boston, MA 02115, USA
To whom correspondence should be addressed
Received July 6, 1995. Revised September 6, 1995. Accepted September 6, 1995.
We have characterized a monoclonal antibody (mAb) to the U1 snRNP component U1 70K. We find that this antibody recognizes several proteins, in addition to U1 70K, in purified spliceosomal complexes and in total HeLa cell nuclear extract preparations. The novel mAb U1 70K antigens can also be specifically immunoprecipitated by the antibody. Similarly to U1 70K, many of the mAb U1 70K antigens can be phosphorylated by a co-purifying kinase activity. The epitope recognized by mAb U1 70K was previously shown to be a repeating arginine/aspartate (RD) dipeptide. Thus we have designated the novel mAb U1 70K antigens the RD family. Comparison of mAb U1 70K with a recently characterized antibody, mAb 16H3, whose epitope is a repeating R/D or R/E motif, showed that a large subset of the antigens are common. In contrast, most of the mAb U1 70K antigens are distinct from the proteins detected by mAb 104, an antibody to the SR family of splicing factors.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. K. Spartz, R. K. Herman, and J. E. Shaw SMU-2 and SMU-1, Caenorhabditis elegans Homologs of Mammalian Spliceosome-Associated Proteins RED and fSAP57, Work Together To Affect Splice Site Choice Mol. Cell. Biol., August 1, 2004; 24(15): 6811 - 6823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. AWASTHI and J. C. ALWINE Association of polyadenylation cleavage factor I with U1 snRNP RNA, November 1, 2003; 9(11): 1400 - 1409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Fortes, D. Bilbao-Cortés, M. Fornerod, G. Rigaut, W. Raymond, B. Séraphin, and I. W. Mattaj Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition Genes & Dev., September 15, 1999; 13(18): 2425 - 2438. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Domon, Z. J. Lorkovic, J. Valcarcel, and W. Filipowicz Multiple Forms of the U2 Small Nuclear Ribonucleoprotein Auxiliary Factor U2AF Subunits Expressed in Higher Plants J. Biol. Chem., December 18, 1998; 273(51): 34603 - 34610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Utz, M. Hottelet, W. J. van Venrooij, and P. Anderson Association of Phosphorylated Serine/Arginine (SR) Splicing Factors With The U1-Small Ribonucleoprotein (snRNP) Autoantigen Complex Accompanies Apoptotic Cell Death J. Exp. Med., February 16, 1998; 187(4): 547 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Taylor, J. T. Nash, E. Theodoridis, A. E. Bygrave, M. J. Walport, and M. Botto A Targeted Disruption of the Murine Complement Factor B Gene Resulting in Loss of Expression of Three Genes in Close Proximity, Factor B, C2, and D17H6S45 J. Biol. Chem., January 16, 1998; 273(3): 1699 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Loyer, J. Trembley, J. Lahti, and V. Kidd The RNP protein, RNPS1, associates with specific isoforms of the p34cdc2-related PITSLRE protein kinase in vivo J. Cell Sci., January 6, 1998; 111(11): 1495 - 1506. [Abstract] [PDF]
|
|