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Nucleic Acids Research, 1995, Vol. 23, No. 21 4234-4238
© 1995

Articles

Nonsense suppressor and antisuppressor mutations at the 1409–1491 base pair in the decoding region of Escherichia coli 16S rRNA Steven

Steven T. Gregory* and Albert E. Dahlberg

Section of Biochemistry, Box G, Brown University, Providence Rl 02912, USA

*To whom correspondence should be addressed

Received September 12, 1995. Accepted September 21, 1995.

Using a genetic selection for suppressors of a UGA nonsense mutation in trpA, we have isolated a G to A transition mutation at position 1491 in the decoding region of 6S rRNA. This suppressor displayed no codon specificity, suppressing UGA, UAG and UAA nonsense mutations and +1 and –1 frameshlft mutations in lacZ. ubsequent examination of a series of mutations at G1491 and its base-pairing partner 409 revealed various effects on nonsense suppression and frameshrfting. Mutations that prevented Watson-Crick base pairing between these residues were observed to increase misreading and frameshiftlng. However, double mutations that retained pairing potential produced an antisuppressor or hyperaccurate phenotype. Previous studies of antibiotic resistance mutations and antibiotic and tRNA footprints have placed G1491 and C1409 near the site of codon-anticodon pairing. The results of this study demonstrate that the nature of the interaction of these two residues influences the fidelity of tRNA selection.


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