Skip Navigation

This Article
Right arrow Print PDF (4998K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Azhayeva, E.
Right arrow Articles by Lönnberg, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Azhayeva, E.
Right arrow Articles by Lönnberg, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 21 4255-4261
© 1995

Articles

Selective binding of looped oligonucleotides to a single-stranded DNA and its influence on replication in vitro

Elena Azhayeva, Alex Azhayev*, Andrei Guzaev and Harri Lönnberg

Department of Chemistry, University of Turku Turku, FIN-20500, Finland

*To whom correspondence should be addressed

Received August 31, 1995. Accepted October 6, 1995.

Complexing of looped and circular oligonucleotides, composed of either 2' deoxyribo or 2-O-metriylribonucteoslde units, with completely matching or partially mismatching complementary DNA sequences was studied. Melting experiments revealed considerable differences among the stabilities of these hybrid complexes. Maximum stability and selectivity was displayed by oligomers 2 and 5. It was concluded that a linear stretch, attached to V-O of 3-deoxypsicothymidine unit (Z) increases the selectivity of hybridisation and stability of the complex as a whole. This allows one to aim the target DNA very precisely at its polyadenine part as well as at adjacent sequence simultaneously. Experiments on termination of primer extension catalysed by different DNA-polymerases Sequenase, Klenow fragment and Tth have demonstrated that looped oligomer 5, composed of 2-O-methylrlbonucleosides appears to be a highly selective and potent inhibitor of replication In vitro. Features of looped oligonucleotides, composed of 2'-O-methylribonucleosides seem to be useful for design of highly specific antigene oligonucleotides.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.