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Nucleic Acids Research, 1995, Vol. 23, No. 21 4290-4295
© 1995

Articles

Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues

Helene F. Rosenberg* and Kimberly D. Dyer

Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda, MD 20892, USA

*To whom correspondence should be addressed

Received August 21, 1995. Accepted September 22, 1995.

We have Isolated a unique genomic fragment encoding human ribonuclease 4 (RNase 4) of the mammalian ribonuclease gene family, whose members Include pancreatic ribonuclease, eosinophil-derived neurotoxin, eosinophll cationic protein and angiogenin. We have determined that the coding sequence of RNase 4 resides on a single exon found on human chromosome 14. The mRNA encoding RNase 4 was detected by Northern analysis In a number of human somatic tissues, including pancreas, lung, skeletal muscle, heart, kidney and placenta, but not brain; liver represents the most abundant source. Interestingly, the mRNA encoding RNase 4 is -2 kb in length, which is approximately twice as large as the mRNAs encoding other members of this gene family. A larger (-2.4 kb), second transcript was detected in hepatic, pancreatic and renal tissues. The ~2 kb RNase 4 mRNA was detected in cells of the human promyelocytlc leukemia line, HL-60, that had been treated with dibutyryl-cAMP to promote neutrophilic differentiation. In contrast, no mRNA encoding RNase 4 could be detected in cells treated with phorbol myristic acid (PMA), an agent promoting differentiation toward monocyte/macrophages, suggesting the existence of elements regulating tissue specific expression of this gene.


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