Nucleic Acids Research, 1995, Vol. 23, No. 21 4407-4414
© 1995
Articles |
AFLP: a new technique for DNA fingerprinting
Keygene N.V. PO Box 216, Wageningen, The Netherlands
*To whom correspondence should be addressed
Received July 14, 1995. Accepted September 5, 1995.
A novel DNA fingerprinting technique called AFLP is described. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA. The technique involves three steps: (i) restriction of the DNA and ligation of oligonucleotide adapters, (ii) selective amplification of sets of restriction fragments, and (iii) gel analysis of the amplified fragments. PCR amplification of restriction fragments is achieved by using the adapter and restriction site sequence as target sites for primer annealing. The selective amplification is achieved by the use of primers that extend into the restriction fragments, amplifying only those fragments in which the primer extensions match the nucleotides flanking the restriction sites. Using this method, sets of restriction fragments may be visualized by PCR without knowledge of nucleotide sequence. The method allows the specific co-amplification of high numbers of restriction fragments. The number of fragments that can be analyzed simultaneously, however, is dependent on the resolution of the detection system. Typically 50—100 restriction fragments are amplified and detected on denaturing polyacrylamide gels. The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. J. Colbeck, H. L. Gibbs, P. P. Marra, K. Hobson, and M. S. Webster Phylogeography of a Widespread North American Migratory Songbird (Setophaga ruticilla) J. Hered., September 1, 2008; 99(5): 453 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mereda Jr., I. Hodalova, P. Martonfi, J. Kucera, and J. Lihova Intraspecific Variation in Viola suavis in Europe: Parallel Evolution of White-flowered Morphotypes Ann. Bot., September 1, 2008; 102(3): 443 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. De Cock, K. Vander Mijnsbrugge, P. Breyne, E. Van Bockstaele, and J. Van Slycken Morphological and AFLP-based Differentiation within the Taxonomical Complex Section Caninae (subgenus Rosa) Ann. Bot., August 23, 2008; (2008) mcn151v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. McDonald, T. L. Parchman, M. R. Bower, W. A. Hubert, and F. J. Rahel An introduced and a native vertebrate hybridize to form a genetic bridge to a second native species PNAS, August 5, 2008; 105(31): 10837 - 10842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Richards, R. L. Walls, J. P. Bailey, R. Parameswaran, T. George, and M. Pigliucci Plasticity in salt tolerance traits allows for invasion of novel habitat by Japanese knotweed s. l. (Fallopia japonica and F.xbohemica, Polygonaceae) Am. J. Botany, August 1, 2008; 95(8): 931 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bory, P. Lubinsky, A.-M. Risterucci, J.-L. Noyer, M. Grisoni, M.-F. Duval, and P. Besse Patterns of introduction and diversification of Vanilla planifolia (Orchidaceae) in Reunion Island (Indian Ocean) Am. J. Botany, July 1, 2008; 95(7): 805 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Kuhl and V. L. DeBoer Genetic Diversity of Rhubarb Cultivars J. Amer. Soc. Hort. Sci., July 1, 2008; 133(4): 587 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kafkas, M. Ozgen, Y. Dogan, B. Ozcan, S. Ercisli, and S. Serce Molecular Characterization of Mulberry Accessions in Turkey by AFLP Markers J. Amer. Soc. Hort. Sci., July 1, 2008; 133(4): 593 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhu, M. Gore, E. S. Buckler, and J. Yu Status and Prospects of Association Mapping in Plants The Plant Genome, July 1, 2008; 1(1): 5 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Ku, Y.-S. Huang, Y.-S. Wang, D. Ma, and K.-W. Yeh IbMADS1 (Ipomoea batatas MADS-box 1 gene) is Involved in Tuberous Root Initiation in Sweet Potato (Ipomoea batatas) Ann. Bot., July 1, 2008; 102(1): 57 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Radoev, H. C. Becker, and W. Ecke Genetic Analysis of Heterosis for Yield and Yield Components in Rapeseed (Brassica napus L.) by Quantitative Trait Locus Mapping Genetics, July 1, 2008; 179(3): 1547 - 1558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Baele, A. Decostere, P. Vandamme, L. Ceelen, A. Hellemans, J. Mast, K. Chiers, R. Ducatelle, and F. Haesebrouck Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs Int J Syst Evol Microbiol, June 1, 2008; 58(6): 1350 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Lubell, M. H. Brand, J. M. Lehrer, and K. E. Holsinger Detecting the influence of ornamental Berberis thunbergii var. atropurpurea in invasive populations of Berberis thunbergii (Berberidaceae) using AFLP1 Am. J. Botany, June 1, 2008; 95(6): 700 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Staelens, D. Rombaut, I. Vercauteren, B. Argue, J. Benzie, and M. Vuylsteke High-Density Linkage Maps and Sex-Linked Markers for the Black Tiger Shrimp (Penaeus monodon) Genetics, June 1, 2008; 179(2): 917 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chappell, C. Robacker, and T. M. Jenkins Genetic Diversity of Seven Deciduous Azalea Species (Rhododendron spp. section Pentanthera) Native to the Eastern United States J. Amer. Soc. Hort. Sci., May 1, 2008; 133(3): 374 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Savo Sardaro, M. Atallah, E. Tavakol, L. Russi, and E. Porceddu Diversity for AFLP and SSR in Natural Populations of Lotus corniculatus L. from Italy Crop Sci., May 1, 2008; 48(3): 1080 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hazzouri, A. Mohajer, S. I. Dejak, S. P. Otto, and S. I. Wright Contrasting Patterns of Transposable-Element Insertion Polymorphism and Nucleotide Diversity in Autotetraploid and Allotetraploid Arabidopsis Species Genetics, May 1, 2008; 179(1): 581 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stokl, P. M. Schluter, T. F. Stuessy, H. F. Paulus, G. Assum, and M. Ayasse Scent variation and hybridization cause the displacement of a sexually deceptive orchid species Am. J. Botany, April 1, 2008; 95(4): 472 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Damborg, L. Guardabassi, K. Pedersen, and B. Kokotovic Comparative Analysis of Human and Canine Campylobacter upsaliensis Isolates by Amplified Fragment Length Polymorphism J. Clin. Microbiol., April 1, 2008; 46(4): 1504 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Saul, M. Krockenberger, and D. Carter Evidence of Recombination in Mixed-Mating-Type and {alpha}-Only Populations of Cryptococcus gattii Sourced from Single Eucalyptus Tree Hollows Eukaryot. Cell, April 1, 2008; 7(4): 727 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jafary, G. Albertazzi, T. C. Marcel, and R. E. Niks High Diversity of Genes for Nonhost Resistance of Barley to Heterologous Rust Fungi Genetics, April 1, 2008; 178(4): 2327 - 2339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. Leitch and M. F. Fay Plant Genome Horizons: Michael Bennett's Contribution to Genome Research Ann. Bot., April 1, 2008; 101(6): 737 - 746. [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Blehert, K. L. Jefferson, D. M. Heisey, M. D. Samuel, B. M. Berlowski, and D. J. Shadduck USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS TO DIFFERENTIATE ISOLATES OF PASTEURELLA MULTOCIDA SEROTYPE 1 J. Wildl. Dis., April 1, 2008; 44(2): 209 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. M. Koopman, V. Wissemann, K. De Cock, J. Van Huylenbroeck, J. De Riek, G. J. H. Sabatino, D. Visser, B. Vosman, C. M. Ritz, B. Maes, et al. AFLP markers as a tool to reconstruct complex relationships: A case study in Rosa (Rosaceae) Am. J. Botany, March 1, 2008; 95(3): 353 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. McKinnon, R. E. Vaillancourt, D. A. Steane, and B. M. Potts An AFLP marker approach to lower-level systematics in Eucalyptus (Myrtaceae) Am. J. Botany, March 1, 2008; 95(3): 368 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Krichen, J. M.S. Martins, P. Lambert, A. Daaloul, N. Trifi-Farah, M. Marrakchi, and J.-M. Audergon Using AFLP Markers for the Analysis of the Genetic Diversity of Apricot Cultivars in Tunisia J. Amer. Soc. Hort. Sci., March 1, 2008; 133(2): 204 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Czarnecki II, M. Nageswara Rao, J. G. Norcini, F. G. Gmitter Jr, and Z. Deng Genetic Diversity and Differentiation among Natural, Production, and Introduced Populations of the Narrowly Endemic Species Coreopsis leavenworthii (Asteraceae) J. Amer. Soc. Hort. Sci., March 1, 2008; 133(2): 234 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vanhaecke, F. Vercruysse, N. Boon, W. Verstraete, I. Cleenwerck, M. De Wachter, P. De Vos, and T. van de Wiele Isolation and Characterization of Human Intestinal Bacteria Capable of Transforming the Dietary Carcinogen 2-Amino-1-Methyl-6-Phenylimidazo[4,5-b]Pyridine Appl. Envir. Microbiol., March 1, 2008; 74(5): 1469 - 1477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kuang, H. J. van Eck, D. Sicard, R. Michelmore, and E. Nevo Evolution and Genetic Population Structure of Prickly Lettuce (Lactuca serriola) and Its RGC2 Resistance Gene Cluster Genetics, March 1, 2008; 178(3): 1547 - 1558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Devey, R. M. Bateman, M. F. Fay, and J. A. Hawkins Friends or Relatives? Phylogenetics and Species Delimitation in the Controversial European Orchid Genus Ophrys Ann. Bot., February 1, 2008; 101(3): 385 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kaga, T. Isemura, N. Tomooka, and D. A. Vaughan The Genetics of Domestication of the Azuki Bean (Vigna angularis) Genetics, February 1, 2008; 178(2): 1013 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xia, Y. Tsubokura, M. Hoshi, M. Hanawa, C. Yano, K. Okamura, T. A. Ahmed, T. Anai, S. Watanabe, M. Hayashi, et al. An Integrated High-density Linkage Map of Soybean with RFLP, SSR, STS, and AFLP Markers Using A Single F2 Population DNA Res, January 11, 2008; (2008) dsm027v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Halverson, S. B. Heard, J. D. Nason, and J. O. Stireman III Origins, distribution, and local co-occurrence of polyploid cytotypes in Solidago altissima (Asteraceae) Am. J. Botany, January 1, 2008; 95(1): 50 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pichot, B. Liens, J. L. R. Nava, J. B. Bachelier, and M. El Maataoui Cypress Surrogate Mother Produces Haploid Progeny From Alien Pollen Genetics, January 1, 2008; 178(1): 379 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Smith, B. M. Wareing, P. C. M. Fogg, L. M. Riley, M. Spencer, M. J. Cox, J. R. Saunders, A. J. McCarthy, and H. E. Allison Multilocus Characterization Scheme for Shiga Toxin-Encoding Bacteriophages Appl. Envir. Microbiol., December 15, 2007; 73(24): 8032 - 8040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mejia, M. Gebauer, L. Munoz, N. Hewstone, C. Munoz, and P. Hinrichsen Identification of QTLs for Seedlessness, Berry Size, and Ripening Date in a Seedless x Seedless Table Grape Progeny Am. J. Enol. Vitic., December 1, 2007; 58(4): 499 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Kalpoe, K. E. Templeton, A. M. Horrevorts, H. P. Endtz, E. J. Kuijper, A. T. Bernards, and C. H. W. Klaassen Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses J. Clin. Microbiol., December 1, 2007; 45(12): 4048 - 4050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Weinthal, I. Barash, M. Panijel, L. Valinsky, V. Gaba, and S. Manulis-Sasson Distribution and Replication of the Pathogenicity Plasmid pPATH in Diverse Populations of the Gall-Forming Bacterium Pantoea agglomerans Appl. Envir. Microbiol., December 1, 2007; 73(23): 7552 - 7561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Kokotovic and O. Angen Genetic Diversity of Actinobacillus pleuropneumoniae Assessed by Amplified Fragment Length Polymorphism Analysis J. Clin. Microbiol., December 1, 2007; 45(12): 3921 - 3929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kashkush and V. Khasdan Large-Scale Survey of Cytosine Methylation of Retrotransposons and the Impact of Readout Transcription From Long Terminal Repeats on Expression of Adjacent Rice Genes Genetics, December 1, 2007; 177(4): 1975 - 1985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chen, Q. Yu, S. Hou, Y. Li, M. Eustice, R. L. Skelton, O. Veatch, R. E. Herdes, L. Diebold, J. Saw, et al. Construction of a Sequence-Tagged High-Density Genetic Map of Papaya for Comparative Structural and Evolutionary Genomics in Brassicales Genetics, December 1, 2007; 177(4): 2481 - 2491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Ariss and G. J. Vandemark Assessment of Genetic Diversity among Nondormant and Semidormant Alfalfa Populations Using Sequence-Related Amplified Polymorphisms Crop Sci., November 7, 2007; 47(6): 2274 - 2284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Maxwell, M. A. Brick, P. F. Byrne, H. F. Schwartz, X. Shan, J. B. Ogg, and R. A. Hensen Quantitative Trait Loci Linked to White Mold Resistance in Common Bean Crop Sci., November 7, 2007; 47(6): 2285 - 2294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Moncada, N. J. Ehlke, G. J. Muehlbauer, C. C. Sheaffer, D. L. Wyse, and L. R. DeHaan Genetic Variation in Three Native Plant Species across the State of Minnesota Crop Sci., November 7, 2007; 47(6): 2379 - 2389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fang, J. Chen, R. J. Henny, and C.-C. T. Chao Genetic Relatedness of Ornamental Ficus Species and Cultivars Analyzed by Amplified Fragment Length Polymorphism Markers J. Amer. Soc. Hort. Sci., November 1, 2007; 132(6): 807 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Andrade, S. J. Mayo, C. van den Berg, M. F. Fay, M. Chester, C. Lexer, and D. Kirkup A Preliminary Study of Genetic Variation in Populations of Monstera adansonii var. klotzschiana (Araceae) from North-East Brazil, Estimated with AFLP Molecular Markers Ann. Bot., November 1, 2007; 100(6): 1143 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Steinmacher, N. G. Krohn, A. C. M. Dantas, V. M. Stefenon, C. R. Clement, and M. P. Guerra Somatic Embryogenesis in Peach Palm Using the Thin Cell Layer Technique: Induction, Morpho-histological Aspects and AFLP Analysis of Somaclonal Variation Ann. Bot., October 1, 2007; 100(4): 699 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Papa, E. Bellucci, M. Rossi, S. Leonardi, D. Rau, P. Gepts, L. Nanni, and G. Attene Tagging the Signatures of Domestication in Common Bean (Phaseolus vulgaris) by Means of Pooled DNA Samples Ann. Bot., October 1, 2007; 100(5): 1039 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Liu, T. Fujita, Z.-H. Yan, S. Sakamoto, D. Xu, and J. Abe QTL Mapping of Domestication-related Traits in Soybean (Glycine max) Ann. Bot., October 1, 2007; 100(5): 1027 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Moon and J. S. Nicholson AFLP and SCAR Markers Linked to Tomato Spotted Wilt Virus Resistance in Tobacco Crop Sci., September 1, 2007; 47(5): 1887 - 1894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Pfaller, T. W. Aronson, A. E. Holtzman, and T. C. Covert Amplified fragment length polymorphism analysis of Mycobacterium avium complex isolates recovered from southern California J. Med. Microbiol., September 1, 2007; 56(9): 1152 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, G. Jin, X. Zhao, Y. Zheng, Z. Xu, and W. Wu PIP: a database of potential intron polymorphism markers Bioinformatics, August 15, 2007; 23(16): 2174 - 2177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Troggio, G. Malacarne, G. Coppola, C. Segala, D. A. Cartwright, M. Pindo, M. Stefanini, R. Mank, M. Moroldo, M. Morgante, et al. A Dense Single-Nucleotide Polymorphism-Based Genetic Linkage Map of Grapevine (Vitis vinifera L.) Anchoring Pinot Noir Bacterial Artificial Chromosome Contigs Genetics, August 1, 2007; 176(4): 2637 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Subramanian, P. Arensburger, P. W. Atkinson, and D. A. O'Brochta Transposable Element Dynamics of the hAT Element Herves in the Human Malaria Vector Anopheles gambiae s.s. Genetics, August 1, 2007; 176(4): 2477 - 2487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. McDaniel, J. H. Willis, and A. J. Shaw A Linkage Map Reveals a Complex Basis for Segregation Distortion in an Interpopulation Cross in the Moss Ceratodon purpureus Genetics, August 1, 2007; 176(4): 2489 - 2500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Herrera-Foessel, R. P. Singh, J. Huerta-Espino, M. William, G. Rosewarne, A. Djurle, and J. Yuen Identification and Mapping of Lr3 and a Linked Leaf Rust Resistance Gene in Durum Wheat Crop Sci., July 30, 2007; 47(4): 1459 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Johnson, T. J. Kisha, and M. A. Evans Characterizing Safflower Germplasm with AFLP Molecular Markers Crop Sci., July 30, 2007; 47(4): 1728 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, H. W. Gao, Y. Q. Wu, and J. G. Han Genetic Diversity and Population Structure of Caragana korshinskii Revealed by AFLP Crop Sci., July 30, 2007; 47(4): 1737 - 1743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
|