Nucleic Acids Research, 1995, Vol. 23, No. 21 4421-4425
© 1995
Articles |
Three subunits of the RNA polymerase II mediator complex are involved in glucose repression
Ludin Institute for Cancer Research, Uppsala Branch, Uppsala Biomedical Center Box 595, S-751 24 Uppsala, Swedan
Received July 7, 1995. Accepted October 5, 1995.
Glucose triggers a complex response in yeast which includes induction and repression of a large number of genes. Glucose repression is in part mediated by the Mlg1 repressor, a zinc finger protein that binds to the promoters of many glucose repressed genes. However, some genes that are required for gluconeogenlc growth are also repressed by a Mig1 -independent mechanism. We have isolated mutations In three genes that are involved In this Mig1-independent component of repression and cloned the genes by complementation. All three genes encode subunits of the recently discovered RNA polymerase II mediator complex. Two of them are yeast cyclin C and its associated kinase. Disruptions of the three genes have identical phenotypes with respect to glucose repression and show no synergism with each other. This suggests that these three subunits of the mediator complex function closely together in transmitting the transcriptional response to glucose.
*Present address:Department of Medical Immunology and Microbiology, Uppsala University, Uppsala Biomedical Center, Box 582, S-751 23 Uppsala, Sweden
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