Skip Navigation

This Article
Right arrow Print PDF (7881K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cairns, C.
Right arrow Articles by McStay, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cairns, C.
Right arrow Articles by McStay, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 22 4583-4590
© 1995

Articles

HMG box 4 is the principal determinant of species specificity in the RNA polymerase I transcription factor UBF

Carol Cairns and Brian McStay*

Biomedical Research Centre, Ninewells Hospital and Medical School, The University of Dundee Dundee DD1 9SY, UK

*To whom correspondence should be addressed

Received September 7, 1995. Accepted October 12, 1995.

Transcription of ribosomal genes requires, in addition to RNA polymerase I, the trans acting factors UBF and Rlb1 in Xenopus or SL1 in humans. RNA polymerase I transcription is remarkably species specific. Between closely related species SL1 Is the sole determinant of this specificity. Between more distantly related species, however, UBF is also a component of this species specificity. Xenopus UBF cannot function in human RNA polymerase I transcription and human UBF cannot function in Xenopus RNA polymerase I transcription. Xenopus and human UBFs are remarkably similar at the amino acid sequence level, both containing multiple HMG box DNA binding motifs. The only major difference between xUBF and hUBF is the lack of a HMG box 4 equivalent in xUBF. Utilizing a series of hybrid UBF molecules we have identified HMG box 4 as the principal determinant of species specificity. Addition of human HMG box 4 to xUBF converts it to a form that functions In human RNA polymerase I transcription. Deletion of HMG box 4 from hUBF converts it to a form that functions in Xenopus RNA polymerase I transcription. Furthermore, mutations within Xenopus UBF demonstrate that UBF requires a precise arrangement and number of HMG boxes to function in RNA polymerase I transcription.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Gonda, J. Wudel, D. Nelson, N. Katoku-Kikyo, P. Reed, H. Tamada, and N. Kikyo
Requirement of the Protein B23 for Nucleolar Disassembly Induced by the FRGY2a Family Proteins
J. Biol. Chem., March 24, 2006; 281(12): 8153 - 8160.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. C. O'Sullivan, G. J. Sullivan, and B. McStay
UBF Binding In Vivo Is Not Restricted to Regulatory Sequences within the Vertebrate Ribosomal DNA Repeat
Mol. Cell. Biol., January 15, 2002; 22(2): 657 - 658.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Y. Stefanovsky, G. Pelletier, D. P. Bazett-Jones, C. Crane-Robinson, and T. Moss
DNA looping in the RNA polymerase I enhancesome is the result of non-cooperative in-phase bending by two UBF molecules
Nucleic Acids Res., August 1, 2001; 29(15): 3241 - 3247.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C Mais and U Scheer
Molecular architecture of the amplified nucleoli of Xenopus oocytes
J. Cell Sci., January 2, 2001; 114(4): 709 - 718.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
H. M. Alzuherri and R. J. White
Regulation of RNA Polymerase I Transcription in Response to F9 Embryonal Carcinoma Stem Cell Differentiation
J. Biol. Chem., February 12, 1999; 274(7): 4328 - 4334.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P Bell, C Mais, B McStay, and U Scheer
Association of the nucleolar transcription factor UBF with the transcriptionally inactive rRNA genes of pronuclei and early Xenopus embryos
J. Cell Sci., January 9, 1997; 110(17): 2053 - 2063.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.