Nucleic Acids Research, 1995, Vol. 23, No. 22 4628-4634
© 1995
Articles |
R4, a non-LTR retrotransponson specific to the large subunit rRNA genes of nematodes
Department of Biology, University of Rochester Rochester, NY 14627 USA 1Institute of Zoology, University of Fribourg Perolles, CH-1700 Fribourg, Switzerland
*To whom correspondence should be addressed
Received August 14, 1995. Accepted October 6, 1995.
A 4.7 kb sequence-specific insertion in the 26S ribosomal RNA gene of Ascaris lumbricoides, named R4, is shown to be a non-long terminal repeat (non- LTR) retrotransposable element. The R4 element inserts at a site in the large subunit rRNA gene which is midway between two other sequence-specific non-LTR retrotransposable elements, R1 and R2, found in most Insect species. Based on the structure of its open reading frame and the sequence of its reverse transcriptase domain, R4 elements do not appear to be a family of R1 or R2 elements that have changed their insertion site. R4 is most similar in structure and in sequence to the element Dong, which is not specialized for insertion into rDNA units. Thus R4 represents a separate non-LTR retrotransposable element that has become specialized for insertion in the rRNA genes of its host. Using oligonucleotide primers directed to a conserved region of the reverse transcriptase encoding domain, Insertions in the R4 site were also amplified from Parascarls equorum and Haemonchus contortus. Why several non-LTR etrotransposable elements have become specialized for insertion into a short (87 bp) region of the large subunit rRNA gene is discussed.
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